Team:KAIT Japan/Notebook

From 2012.igem.org

(Difference between revisions)
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='''Notebook'''=
='''Notebook'''=
=='''Creating parts of Tar methylation region'''==
=='''Creating parts of Tar methylation region'''==
-
===Date:8/9===
+
:<big>'''Date:8/9'''</big>
-
Colony PCR
+
::Colony PCR
-
::'''→Reflection:We Took E.coli too many.You should take less E.coli.'''<br>
+
:::'''→Reflection:We Took E.coli too many.You should take less E.coli.'''<br>
-
::'''→Reflection:The number of cycles was less.So,We increased The number of cycles in 8/11.(50 cycles) '''
+
:::'''→Reflection:The number of cycles was less.So,We increased The number of cycles in 8/11.(50 cycles) '''
-
----
+
:<big>'''Date:8/11'''</big>
 +
::The purified DNA
 +
:::'''→Reflection:Band was less.'''
 +
::PCR Product
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===Date:8/11===
+
:<big>'''Date:8/13'''</big>
-
The purified DNA
+
::Confirmed of electrophoresis by PCR product and Ligation of the TA vector
-
::'''→Reflection:Band was less.'''
+
-
PCR Product
+
-
----
+
:<big>'''Date:8/14'''</big>
 +
::Transformation
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===Date:8/13===
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:<big>'''Date:8/25'''</big>
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Confirmed of electrophoresis by PCR product and Ligation of the TA vector
+
::Miniprep
-
 
+
-
----
+
-
 
+
-
===Date:8/14===
+
-
Transformation
+
-
 
+
-
----
+
-
 
+
-
===Date:8/25===
+
-
Miniprep
+
=='''Creating parts of azurin'''==
=='''Creating parts of azurin'''==
-
===Date:8/16===
+
:<big>'''Date:8/16'''</big>
-
Colony PCR
+
::Colony PCR
-
Ligation
+
::Ligation
-
 
+
-
----
+
-
 
+
-
===Date:8/18===
+
-
Colony PCR
+
-
----
+
:<big>'''Date:8/18'''</big>
 +
::Colony PCR
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===Date:8/20===
+
:<big>'''Date:8/20'''</big>
-
DNA extraction and purification of ''P.aeruginosa''
+
::DNA extraction and purification of ''P.aeruginosa''
|}
|}

Revision as of 04:30, 13 September 2012

KAIT Japan2012 logo.png
KAIT Japan2012 Sub.png
Kaitjapan iGEM official.logo.png


Kaitjapan.home.png

Home

Kaitjapan project.png

Project

Kaitjapan parts.png

Parts

Kaitjapan protocol.png

Protocol

Kaitjapan notebook.png

Notebook

Kaitjapan results.png

Results

Kaitjapan safety.png

Safety

Kaitjapan human practice.png

Human Practice

Kaitjapan team.png

Team

Notebook

Creating parts of Tar methylation region

Date:8/9
Colony PCR
→Reflection:We Took E.coli too many.You should take less E.coli.
→Reflection:The number of cycles was less.So,We increased The number of cycles in 8/11.(50 cycles)
Date:8/11
The purified DNA
→Reflection:Band was less.
PCR Product
Date:8/13
Confirmed of electrophoresis by PCR product and Ligation of the TA vector
Date:8/14
Transformation
Date:8/25
Miniprep

Creating parts of azurin

Date:8/16
Colony PCR
Ligation
Date:8/18
Colony PCR
Date:8/20
DNA extraction and purification of P.aeruginosa
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