Team:LMU-Munich/Germination Stop

From 2012.igem.org

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Two methods are being employed to knock out germination: resistance cassette (RC) knockouts and clean deletions. Single RC knockouts were created first; then they were combined to create multiple knockouts.
Two methods are being employed to knock out germination: resistance cassette (RC) knockouts and clean deletions. Single RC knockouts were created first; then they were combined to create multiple knockouts.
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[[File:germination_gene_knockouts_resized.jpg|frame|center|Fig. 1: The four germination genes being knocked out, and their resistance cassette replacements, as shown on the ''Bacillus'' chromosome.]]
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[[File:germination_gene_knockouts.jpg|Fig. 1: The four germination genes being knocked out, and their resistance cassette replacements, as shown on the ''Bacillus'' chromosome.|thumb|610px]]
[[File:LFH_image.jpg|Fig. 2: The technique employed for resistance cassette knockouts: Long-flanking-homology PCR. This example shows the gene ''cwlD'' being replaced with the resistance cassette kanamycin.|thumb|610px]]
[[File:LFH_image.jpg|Fig. 2: The technique employed for resistance cassette knockouts: Long-flanking-homology PCR. This example shows the gene ''cwlD'' being replaced with the resistance cassette kanamycin.|thumb|610px]]

Revision as of 11:36, 12 September 2012

iGEM Ludwig-Maximilians-Universität München Beadzillus

Team-LMU streaked plate.resized.jpg

The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".

IGEM HQ LMU prize.jpg

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