Team:TU Darmstadt/Materials/T4 DNA Ligase

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Revision as of 19:24, 11 September 2012

Contents

T4 DNA Ligase

About

T4 DNA ligase is an enzyme that facilitates the joining of DNA stands by catalyzing phosphodiester bonds. It was isolated from [http://en.wikipedia.org/wiki/Enterobacteria_phage_T4 bacteriophage T4] and is used to generate recombinant DNA sequences.[1]

Working conditions

For ligating extended oligos in a PCR reaction it is necessary to have phosphorilated 5'-ends. Oligos will only be ligated when they are assembled on a compementary strand. [2]

Supplier

  • NEB http://www.neb.com/nebecomm/products/productR3133.asp

References

[1] http://en.wikipedia.org/wiki/DNA_ligase

[2] http://openwetware.org/wiki/Thermostable_DNA_ligase

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