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Team:Macquarie Australia/Protocols/ResultsofGibsonsofar110912
From 2012.igem.org
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==='''Results'''=== | ==='''Results'''=== | ||
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| - | * T7 Heme oxygenase (chloramphenicol): This biobrick assembly was successful. The plasmids will be sequenced. | + | * T7 Heme oxygenase (chloramphenicol): This biobrick assembly was successful. The plasmids will be sequenced and re-transformed into BL21 DE3 Rosetta & Star cells. |
* Heme oxygenase (Kanamycin): Incubation is to be performed today and plasmid preparation using the Qiagen protocol will be performed. No colonies were present on the T7 minus heme oxygenase on ampicillin. | * Heme oxygenase (Kanamycin): Incubation is to be performed today and plasmid preparation using the Qiagen protocol will be performed. No colonies were present on the T7 minus heme oxygenase on ampicillin. | ||
* Deinococcus: Plasmid sequencing will be performed and transformation was successful on kanamycin plates. The Deinococcus on the kanamycin was unsuccessful. | * Deinococcus: Plasmid sequencing will be performed and transformation was successful on kanamycin plates. The Deinococcus on the kanamycin was unsuccessful. | ||
* T7 Agrobacterium: no colonies were present on the chloramphenicol plates. | * T7 Agrobacterium: no colonies were present on the chloramphenicol plates. | ||
* Agrobacterium: no colonies were present on either Kanamycin or Ampicillin plates. | * Agrobacterium: no colonies were present on either Kanamycin or Ampicillin plates. | ||
Revision as of 02:44, 11 September 2012
Results
- T7 Heme oxygenase (chloramphenicol): This biobrick assembly was successful. The plasmids will be sequenced and re-transformed into BL21 DE3 Rosetta & Star cells.
- Heme oxygenase (Kanamycin): Incubation is to be performed today and plasmid preparation using the Qiagen protocol will be performed. No colonies were present on the T7 minus heme oxygenase on ampicillin.
- Deinococcus: Plasmid sequencing will be performed and transformation was successful on kanamycin plates. The Deinococcus on the kanamycin was unsuccessful.
- T7 Agrobacterium: no colonies were present on the chloramphenicol plates.
- Agrobacterium: no colonies were present on either Kanamycin or Ampicillin plates.
