Team:Macquarie Australia/Protocols/ResultsofGibsonsofar110912

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Results from sequencing at the Macquarie facility are as follows:
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* T7 Heme oxygenase (chloramphenicol): This biobrick assembly was successful. The plasmids will be sequenced.
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* Heme oxygenase (Kanamycin): Incubation is to be performed today and plasmid preparation using the Qiagen protocol will be performed. No colonies were present on the T7 minus heme oxygenase on ampicillin.
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* Deinococcus: Plasmid sequencing will be performed and transformation was successful on kanamycin plates. The Deinococcus on the kanamycin was unsuccessful.
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* T7 Agrobacterium: no colonies were present on the chloramphenicol plates.
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* Agrobacterium: no colonies were present on either Kanamycin or Ampicillin plates.

Revision as of 02:42, 11 September 2012




Results from sequencing at the Macquarie facility are as follows:

  • T7 Heme oxygenase (chloramphenicol): This biobrick assembly was successful. The plasmids will be sequenced.
  • Heme oxygenase (Kanamycin): Incubation is to be performed today and plasmid preparation using the Qiagen protocol will be performed. No colonies were present on the T7 minus heme oxygenase on ampicillin.
  • Deinococcus: Plasmid sequencing will be performed and transformation was successful on kanamycin plates. The Deinococcus on the kanamycin was unsuccessful.
  • T7 Agrobacterium: no colonies were present on the chloramphenicol plates.
  • Agrobacterium: no colonies were present on either Kanamycin or Ampicillin plates.