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From 2012.igem.org

Revision as of 12:43, 29 August 2012 (view source) RussellGritton (Talk | contribs) (Created page with "{{UEANRP}} {{UEANRPLabs}} =Week 10= ==Day 1== ==Day 2== ==Day 3== ==Day 4== ==Day 5==") ← Older edit		Revision as of 17:21, 10 September 2012 (view source) Khadijaouadi (Talk | contribs) (→Day 1) Newer edit →
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	==Day 1==		==Day 1==
		+
		+	- Backup comparator circuit mini preps and digest with Eco R1 and Pst1 (overnight)
		+
		+	- BM minipreped (didn't work so re-inoculated)
		+
		+	- Digest MB samples with Spe1 and Pst1, cut out and purify
		+
		+	- CFP and RFP digested with PstI and XbaI (cut out and gell purified)
		+
		+	- Ligate MB2, MB3,MB5,MB9,MB10 with RFP/CFP (overnight)
		+
		+	- Ligate Comparator insert with PSB1C3 (overnight)
	==Day 2==		==Day 2==

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Revision as of 17:21, 10 September 2012

Welcome to the NRP UEA iGEM 2012 Wiki Lab Book

Please choose the relevant link to access our diary of that week!

Week Zero | Week One | Week Two | Week Three | Week Four | Week Five | Week Six | Week Seven | Week Eight | Week Nine | Week Ten | Week Eleven | Lab Protocols | Experiments

Contents 1 Week 10 1.1 Day 1 1.2 Day 2 1.3 Day 3 1.4 Day 4 1.5 Day 5

Week 10

Day 1

- Backup comparator circuit mini preps and digest with Eco R1 and Pst1 (overnight)

- BM minipreped (didn't work so re-inoculated)

- Digest MB samples with Spe1 and Pst1, cut out and purify

- CFP and RFP digested with PstI and XbaI (cut out and gell purified)

- Ligate MB2, MB3,MB5,MB9,MB10 with RFP/CFP (overnight)

- Ligate Comparator insert with PSB1C3 (overnight)

Day 2

Day 3

Day 4

Day 5