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Team:Uppsala University/Results
From 2012.igem.org
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<p><b>New standard backbones</b></p> | <p><b>New standard backbones</b></p> | ||
| + | <p>We have constructed a range of new standard backbones, especially low copy backbones and backbones with built-in lacIq repression for tight control of toxic genes. This work was induced as it turned out that the common registry pSB4 backbones all have a faulty copy number regulation, while we needed low copy backbones for out project. <a href="http://https://2012.igem.org/Team:Uppsala_University/Backbones"> Read more...</a></p> | ||
<p><b>Scarless gene deletion</b></p> | <p><b>Scarless gene deletion</b></p> | ||
| + | <p>In our project we have extensivly used recombineering onto the bacterial chromosome. To aid this work, we have built a improved Cat-SacB selection-counterselection casette, which provides very easy and reliable recombineering and the possibility of scarless gene deletion. <a href="http://https://2012.igem.org/Team:Uppsala_University/Catsac"> Read more...</a></p> | ||
<p><b>Promoter charaterization</b></p> | <p><b>Promoter charaterization</b></p> | ||
<p><b>Chromoproteins</b></p> | <p><b>Chromoproteins</b></p> | ||
Revision as of 05:52, 10 September 2012
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New standard backbones We have constructed a range of new standard backbones, especially low copy backbones and backbones with built-in lacIq repression for tight control of toxic genes. This work was induced as it turned out that the common registry pSB4 backbones all have a faulty copy number regulation, while we needed low copy backbones for out project. Read more... Scarless gene deletion In our project we have extensivly used recombineering onto the bacterial chromosome. To aid this work, we have built a improved Cat-SacB selection-counterselection casette, which provides very easy and reliable recombineering and the possibility of scarless gene deletion. Read more... Promoter charaterization Chromoproteins |
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