Team:MIT/MaterialsAndMethods

From 2012.igem.org

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<h1> Gate Anneals </h1>
<h1> Gate Anneals </h1>
<u> Room Temperature Annealing </u>
<u> Room Temperature Annealing </u>
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<OL>&nbsp;
+
<OL>
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<LI>Mix gate and output in a 1.2:1 ratio in a tube.</LI>
+
<LI>&nbsp;Mix gate and output in a 1.2:1 ratio in a tube.</LI>
-
<LI>Cover the tube in foil, if you didn't use an opaque/black tube.</LI>
+
<LI>&nbsp;Cover the tube in foil, if you didn't use an opaque/black tube.</LI>
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<LI>Leave the tube on a bench top overnight.</LI>
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<LI>&nbsp;Leave the tube on a bench top overnight.</LI>
</OL>
</OL>

Revision as of 22:52, 9 September 2012

iGEM 2012

Bacterial

  • Placeholder1
  • Placeholder2

In Vitro

  • Gate Anneals
  • Plate Reader Studies

Mammalian

  • DNA Transfection
  • RNA Transfection

Gate Anneals

Room Temperature Annealing
  1.  Mix gate and output in a 1.2:1 ratio in a tube.
  2.  Cover the tube in foil, if you didn't use an opaque/black tube.
  3.  Leave the tube on a bench top overnight.

Plate Reader Studies

PLACEHOLDER TEXT

RNA Transfection

PLACEHOLDER TEXT

RNA Transfection

PLACEHOLDER TEXT