Team:LMU-Munich/Laboratory Safety

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Revision as of 14:43, 7 September 2012

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Lab and Project Safety

For iGEM 2012, teams are asked to detail how they approached any issues of biological safety associated with their projects. Specifically, teams should consider the following questions:

1) Would any of your project ideas raise safety issues in terms of:

• a) researcher safety,
• b) public safety, or
• c) environmental safety?

2) Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues? If yes,

• a) did you document these issues in the Registry?
• b) how did you manage to handle the safety issue?
• c) How could other teams learn from your experience?

3) Is there a local biosafety group, committee, or review board at your institution?

• a) If yes, what does your local biosafety group think about your project?
• b) If no, which specific biosafety rules or guidelines do you have to consider in your country?

4) Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering?

Answers:

In general:

To assure safe working practice throughout the competition, every team member participated in a general safety meeting regarding good laboratory practice and working with genetically modified organisms (GMOs), including storage and disposal. We work only with non-hazardous, non-pathogenic organisms like Escherichia coli (lab strain XL1 blue) and Bacillus subtilis (W168). We follow the safety regulations that apply for the [http://en.wikipedia.org/wiki/Biosafety_level#Biosafety_level_1 biological safety level 1] classification. That means we wear a lab coat and single-use gloves. When working with hazardous chemicals (e.g. liquid N₂) we wear goggles as well. Furthermore, dangerous substances are stored and handled in designated rooms in order to assure the safety of the researchers.

For the protection of the public and the environment against hazardous substances, all GMO-contaminated waste is inactivated by autoclavation. Before leaving the laboratory, every researcher cleans and disinfects his/her hands. Moreover, we leave the windows closed and do not discard any dangerous substances in the sink.

Question 1

The subproject BacillusBioBrickBox is about the construction and evaluation of new BioBricks for the work with B. subtilis (see Answer 2) and therefore does not raise any safety issues.

The other subproject, Sporobead involves potentially the creation of GMOs and could have unknown effects to the public or the environment. Although we have great plans for the use of our Sporobeads, they never leave our laboratory, so they cannot harm the public or environment. For possible future applications, we try to block the germination of our Sporobeads in two different ways (GerminationStop), so that they cannot proliferate. This is our approach towards the safety of our Sporobeads.

We have not worked on it yet, but there also is the possibility to remove antibiotic resitance genes from the Bacillus subtilis genome (for example with pMAD, a vector that we used to delete genes in B. subtilis). All our constructs are used while being integrated into the genome. Therefore all resistances could be removed (without removing the functional constructs) before the use of the Sporobeads. Thereby any pathogens would not have the possibility to grab resistance genes from our strains.

From the best of our knowledge the parts, strains or spores we use do not raise any safety concerns. However a point that needs to be discussed here is the potential risk of misusing our spores. We offer an easy platform for displaying any protein of interest. Could this platform be used by e.g. a bio-terrorist? Since all information on how to build a spore with a protein of interest is given on this website one could imagine to use Sporobeads as a vehicle for lethal proteins. Our team is aware of this potential risk. However, our spores can not germinate, so the risk of a rapidly spreading epidemic is negligible. Today it still seems "easier" to use directly know pathogens like e.g. Helicobacter pylori.

Question 2

Our biobricks contain promotors, regulators, a bacterial toxine and reporter genes. None of them are able to cause illnesses or threaten humans in any other way. All inserts are also derived from non-pathogenic, non-hazardous organisms. The amplified and cloned fragments again belong to the GMO safety class S1.

Question 3

We did an interview with the safety Commissioner Dr. Schubar who is responsible for our university. He confirmed that we are working with a safe strain (B. subtilis W168 which has a tryptophan auxotrophy) and are only using safe plasmids, genes and promoters. He is not in a position to allow the release of our spores, but according to the present law, it should be allowed. At the moment, there are ongoing discussions for a SynBio law which is not established, yet. For details, please have a look at our interview.

Germany has signed and ratified the [http://www.cbd.int/doc/legal/cartagena-protocol-en.pdf Cartagena Biosafety Protocol]. This protocol ensures safe handling, use and transfer of genetically modified organisms. Furthermore, we have our own laws and guidelines for biosafety here. For example, all laboratories which are handling GMOs have a designated biosafety level, which is stated in a genetic engineering decree ([http://www.gesetze-im-internet.de/bundesrecht/gentsv/gesamt.pdf Gentechnik Sicherheitsverordnung]) and monitored by university officials.

The general safety rules are listed here (This file is derived from Göttingen University, but the rules are identical.)

Question 4

One part is the removal of resistance cassettes (not possible in organsims that have plasmids). We also like the toxin-antitoxin system described by Cambridge last year.

Part of the GerminationSTOP is the Suicideswitch (see GerminationSTOP) which yields a toxin during Sporulation and therefore kills the cell upon germination. This is a device dedicated to make our Sporobeads safe. But if linked to another Promotor it could be turned on in other cases and could therefore also make other systems safer.