Team:Technion/3 September 2012

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==Rachel==
==Rachel==
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Today I cleaned the PCR product of K1F RNAP from the gel. The concentration was: 17 ng/ul. <br>
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-Clean the PCR product of K1F RNAP (the one with pPROLAR primers)from the gel. The concentration was: 17 ng/ul. <br>-Digest of K1F RNAP with HindIII and NHeI for creating sticky ends.<br>-Clean the K1F RNAP after digestio by cleaning kit. The concentration was: 25 ng/ul

Revision as of 13:34, 7 September 2012

{{:Team:Technion/Template:Notebook|content=

Ilya

- Miniprep of the low copy number pCP plasmid.
- PCR of Fus2 and Fus2del from pSB3C5.

Inbal

- Mini-prep for the 3 starters I put yesterday. The concentrations are: 115, 197 and 150 ng/ul! :] (at least I know how to mini prep like a pro!)
- 4 starters of BBa_B0015 ( the insert is double terminators, the size is 2200bp roughly).
- Cleaning from gel the T7* RNAP (the one with pSB1A2 primers), the concentrations were l-o-w: about 7.5ng/ul after calculating it from just looking at the band's intensity (cause the NanoDrop wasn't accurate).
- clean the pSB1C3 plasmid after digestion with XbaI and PstI by cleaning kit.

Asaf

I cleaned the PCR products of the polymerase genes from the gel.

Hila

PCR reaction for fragments 1, 1C, 6, 6C using T = 55, 58, 60.
Cross your fingers…

Restriction with PacI and SpeI for fragments 2C, 3C, 4C, 5C, 7C, 8C and the confirmed MCS in the pSB1C3+MCS.

Lior

Noa

Evgeni

- No growth of starters again!!! Probably I'm doing some stupid mistake during their preparation, Orna will watch over me today during starters' preparation.

Shahar

Rachel

-Clean the PCR product of K1F RNAP (the one with pPROLAR primers)from the gel. The concentration was: 17 ng/ul.
-Digest of K1F RNAP with HindIII and NHeI for creating sticky ends.
-Clean the K1F RNAP after digestio by cleaning kit. The concentration was: 25 ng/ul

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