Team:LMU-Munich/Germination Stop

From 2012.igem.org

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[[File:LMU SuicideSwitch grafik.png|700px|thumb|center]]
[[File:LMU SuicideSwitch grafik.png|700px|thumb|center]]
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To have a backup plan to make our <b>Sporo</b>beads even safer, we developed the <b>Suicide</b>switch. In case the spores do germinate, because there crust is harmed in any other way, for example high pressure, the <b>Suicide</b>switch will be turned on. It is composed by an alternative sigma factor ecf41 turned on by a sigma G regulated promotor responding quite late to sigma G (PspoIVB responding strongly or PsspK responding weakly) which is the last sigma factor activated in the forespore. Ecf41 then activates the PydfG promotor which then activates the transcription of MazF, a bacterial toxin degrading mRNA.
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The idea behind this is to pack the <b>Sporo</b>beads full with ecf41 when they sporulate, which will in turn kill them upon germination due to the MazF.
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We chose MazF, as we think it won't be able to harm our <b>Sporo</b>bead even if it is made too early, as the spore doesn't rely on translation to be preserved.
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We are planning to model this system.

Revision as of 17:19, 8 September 2012

iGEM Ludwig-Maximilians-Universität München Beadzillus

Team-LMU streaked plate.resized.jpg

The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".

IGEM HQ LMU prize.jpg

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