Team:Tec-Monterrey/antifreeze/project

From 2012.igem.org

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<p>Our project is based in the development of a standard production and expression of allergen proteins (from maize, dustmite and bees) in order for them to be easily purified and used in a new diagnostic kit designed for the detection of allergies.</p>
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<p>This project's objective is the development of a new E. coli strain capable of surviving numerous steps of freezing and thawing, due to the expression of antifreeze proteins from the beetlle Rhagium inquisitor (RiAFP). We expect this new strain to be easier to handle in research labs and to increase the overall efficiency of transformed cells.</p>
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<p>The project involves the construction of device that would allow the expression of allergen proteins in yeast, resulting in the secretion of the proteins to the medium, while also maintaining the ability to also produce the proteins in E. coli. The design of the kit is based on an ELISA assay and includes the production of modified scFv’s (single-chain variable fragment) that allow the detection of possible allergic reactions in an individual.</p>
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<p>We plan to modify this strain's chromosomal DNA by Red/ET homologous recombination, and to insert the neccesary sequences for the expression of the RiAFP in the bacteria's cytoplasmic and periplasmic spaces. Furthermore, we will use an experimental design to find out the best storing conditions for this strain during cryopreservation.</p>
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<p>Our project is based in the development of a standard production and expression of allergen proteins (from maize, dustmite and bees) in order for them to be easily purified and used in a new diagnostic kit designed for the detection of allergies.</p>
+
<p>This project's objective is the development of a new E. coli strain capable of surviving numerous steps of freezing and thawing, due to the expression of antifreeze proteins from the beetlle Rhagium inquisitor (RiAFP). We expect this new strain to be easier to handle in research labs and to increase the overall efficiency of transformed cells.</p>
-
<p>The project involves the construction of device that would allow the expression of allergen proteins in yeast, resulting in the secretion of the proteins to the medium, while also maintaining the ability to also produce the proteins in E. coli. The design of the kit is based on an ELISA assay and includes the production of modified scFv’s (single-chain variable fragment) that allow the detection of possible allergic reactions in an individual.</p>
+
<p>We plan to modify this strain's chromosomal DNA by Red/ET homologous recombination, and to insert the neccesary sequences for the expression of the RiAFP in the bacteria's cytoplasmic and periplasmic spaces. Furthermore, we will use an experimental design to find out the best storing conditions for this strain during cryopreservation.</p>
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Revision as of 03:14, 7 September 2012

Tec Igem 2012 1 2 3 4 5 6 7 8 9 10 11 12

This project's objective is the development of a new E. coli strain capable of surviving numerous steps of freezing and thawing, due to the expression of antifreeze proteins from the beetlle Rhagium inquisitor (RiAFP). We expect this new strain to be easier to handle in research labs and to increase the overall efficiency of transformed cells.

We plan to modify this strain's chromosomal DNA by Red/ET homologous recombination, and to insert the neccesary sequences for the expression of the RiAFP in the bacteria's cytoplasmic and periplasmic spaces. Furthermore, we will use an experimental design to find out the best storing conditions for this strain during cryopreservation.

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Our pro.

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°....Our pro.

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Our pro.

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Our pro.

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Our pro.
project

This project's objective is the development of a new E. coli strain capable of surviving numerous steps of freezing and thawing, due to the expression of antifreeze proteins from the beetlle Rhagium inquisitor (RiAFP). We expect this new strain to be easier to handle in research labs and to increase the overall efficiency of transformed cells.

We plan to modify this strain's chromosomal DNA by Red/ET homologous recombination, and to insert the neccesary sequences for the expression of the RiAFP in the bacteria's cytoplasmic and periplasmic spaces. Furthermore, we will use an experimental design to find out the best storing conditions for this strain during cryopreservation.

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