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Team:TU Darmstadt/Protocols
From 2012.igem.org
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Protein_structure_prediction Protein structure prediction] | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Protein_structure_prediction Protein structure prediction] | ||
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/RNA_secondary_structure_prediction RNA secondary structure prediction] | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/RNA_secondary_structure_prediction RNA secondary structure prediction] | ||
| + | |||
| + | == Material Science == | ||
| + | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Synthesis_of_paranitrophenylesters_with_acyl_chlorides_in_presence_of_triethylamine Synthesis of paranitrophenylesters with acyl chlorides in presence of triethylamine | ||
== Miscellaneous == | == Miscellaneous == | ||
Revision as of 12:00, 7 September 2012
The following page(s) gives an overview of the protocols and possible modifications that where used and proven working during the iGEM PET.erminators project 2012.
Contents |
In vivo
[http://en.wikipedia.org/wiki/E._coli Escherichia coli]
- Bacterial cell culture
- Bacterial transformation aka TRAFO
- Cell counting/plating
- Chemically competent cells
- Colony PCR
- Electrocompetent cells
- Electroporation
- Engineering BioBrick vectors from BioBrick parts/Colony PCR
- In-fusion BioBrick assembly
- Miniprep / Qiagen kit
- Transformation of chemically competent cells
- Western blot
Comamonas testosteroni
In vitro
Nucleic acids
[http://en.wikipedia.org/wiki/DNA DNA]
- Agarose gel electrohporesis
- Annealing and primer extension
- Annealing primers
- Assembly PCR
- Cloning
- DNA Ligation
- DNA Quantification / NanoDrop
- DNA Synthesis from Oligos
- Engineering BioBrick vectors from BioBrick parts/Colony PCR
- Miniprep
- [http://en.wikipedia.org/wiki/Polymerase_chain_reaction PCR]
- Polyacrylamide gel electrophoresis
- Primer Design
- Purification of DNA
- Restriction digest
- Resuspension of primers
- Salting Out
- Sequencing DNA
[http://en.wikipedia.org/wiki/RNA RNA]
Protein
In silico
Data analysis
Databases
Modelling
Sequence analysis
Structure analysis
Material Science
- [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Synthesis_of_paranitrophenylesters_with_acyl_chlorides_in_presence_of_triethylamine Synthesis of paranitrophenylesters with acyl chlorides in presence of triethylamine
Miscellaneous
Cell culture
Microscopy
ILDs / Improvised Lab Devices
- x bp Primer Designer
- Enhancing a thermo cyclers cooling capabilites
- Gel pocket size reduction
- Growing iGEMlers
