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Team:SDU-Denmark/labwork/Notebook/week2
From 2012.igem.org
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<h2>PCR of SST and FFT</h2> <br/> | <h2>PCR of SST and FFT</h2> <br/> | ||
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We did a <a href="https://2012.igem.org/Team:SDU-Denmark/labwork/Protocols/PCR"><b>polymerase chain reaction</b></a> with two different settings; one where we used the recommended settings from the protocol and one where we specified the temperature for each cycle in the PCR which should be more optimal for the primers. <br> | We did a <a href="https://2012.igem.org/Team:SDU-Denmark/labwork/Protocols/PCR"><b>polymerase chain reaction</b></a> with two different settings; one where we used the recommended settings from the protocol and one where we specified the temperature for each cycle in the PCR which should be more optimal for the primers. <br> | ||
The genomic template we used was MCF7. The PCR samples were processed over night (O.N.). | The genomic template we used was MCF7. The PCR samples were processed over night (O.N.). | ||
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Revision as of 14:01, 6 September 2012
Laboratory Notebook
Here you find the log book for the procedures carried out in the laboratory, starting from week 27.
09-07-2012
PCR of SST and FFT
We did a polymerase chain reaction with two different settings; one where we used the recommended settings from the protocol and one where we specified the temperature for each cycle in the PCR which should be more optimal for the primers.
The genomic template we used was MCF7. The PCR samples were processed over night (O.N.).
