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Revision as of 09:34, 5 September 2012 (view source) Ehoyer (Talk | contribs) ← Older edit		Revision as of 09:35, 5 September 2012 (view source) Ehoyer (Talk | contribs) Newer edit →
Line 21:		Line 21:
	| 2µl Hemo_T7A		| 2µl Hemo_T7A
	|-		|-
-	! scope="row"| Heme oxygenase (T7 minus)	+	! scope="row"|
-	| Hemo_A + Hemo_B + Plasmid	+	| 2µl Hemo_B
		+
	|-		|-
	! scope="row"| T7 Agrobacterium		! scope="row"| T7 Agrobacterium

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Revision as of 09:35, 5 September 2012

Gibson Assembly Protocol

Before entering the lab, the assigned Gibson Assembly team for the day organised agar plate requirements, concentration calculations and equipment requirements.

Instruction	#1C	#2K	#2A	#3K	#3A	#4C	#5K	#5A
Addition of 20ng Gene Block Fragments in appropriate tube	2µl Hemo_T7A
	2µl Hemo_B
T7 Agrobacterium	Agro_T7_A + Agro_B + Agro_C + Agro_D + Agro_E + Plasmid
Agrobacterium (T7 minus)	Agro_A + Agro_B + Agro_C + Agro_D + Agro_E + Plasmid Performing Gibson Assembly Planning lab work for the day Calculations