Team:LMU-Munich/Laboratory Safety

From 2012.igem.org

(Difference between revisions)
Line 39: Line 39:
<b>Question 1</b>
<b>Question 1</b>
-
The subproject <b><i>Bacillus</i>B</b>io<b>B</b>rick<b>B</b>ox is about the construction and evaluation of harmless BioBricks (see Answer 2) and therefore does not raise any safety issues. The Sporobeads are potentially living GMOs and could potentially harm the public or the environment. Although we have great plans for the use of our Sporobeads, they never leave our laboratory, so they cannot harm the public or environment. For possible future applications, we try to block the germination of our Sporobeads so that they can not proliferate in two different ways (see [[Team:LMU-Munich/Germination_Stop germination stop]]). This is our approach towards the safety of our Sporobeads.  
+
The subproject <b><i>Bacillus</i>B</b>io<b>B</b>rick<b>B</b>ox is about the construction and evaluation of harmless BioBricks (see Answer 2) and therefore does not raise any safety issues. The Sporobeads are potentially living GMOs and could potentially harm the public or the environment. Although we have great plans for the use of our Sporobeads, they never leave our laboratory, so they cannot harm the public or environment. For possible future applications, we try to block the germination of our Sporobeads so that they can not proliferate in two different ways (see [[Team:LMU-Munich/Germination_Stop '''Germination'''Stop]]). This is our approach towards the safety of our Sporobeads.  
We did not work on that, but there also is the possibility to remove antibiotic resitance genes from the Bacillus subtilis genome [for example with [https://static.igem.org/mediawiki/2012/6/6d/LMU-Munich_2012_Clean_deletions_in_Bacillus_subtilis.pdf pMAD], a vector that we used to delete genes in ''B. subtilis'']. All our construct are used while being integrated into the genome. Therefore all resistances could be removed (without removing the functional constructs) before the use of the Sporobeads. Thereby any pathogens would not have the possibility to grab resistance genes from our strains.
We did not work on that, but there also is the possibility to remove antibiotic resitance genes from the Bacillus subtilis genome [for example with [https://static.igem.org/mediawiki/2012/6/6d/LMU-Munich_2012_Clean_deletions_in_Bacillus_subtilis.pdf pMAD], a vector that we used to delete genes in ''B. subtilis'']. All our construct are used while being integrated into the genome. Therefore all resistances could be removed (without removing the functional constructs) before the use of the Sporobeads. Thereby any pathogens would not have the possibility to grab resistance genes from our strains.

Revision as of 09:45, 5 September 2012

iGEM Ludwig-Maximilians-Universität München Beadzillus

Team-LMU agar plates.resized.jpg

The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".

IGEM HQ LMU prize.jpg

[ more news ]

Sporenfreunde