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Team:Macquarie Australia/Protocols/CompetentCellTesting
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==='''Competent Cell Testing'''=== | ==='''Competent Cell Testing'''=== | ||
| - | Results: | + | '''Results:''' |
* The transformation efficiency for all of the Top10 cells were 1 x 10^7 CFU/µg while the transofmration efficiency for the Fusion Blue and BL21 cells were 1 x 10^6 CFU/µg. | * The transformation efficiency for all of the Top10 cells were 1 x 10^7 CFU/µg while the transofmration efficiency for the Fusion Blue and BL21 cells were 1 x 10^6 CFU/µg. | ||
Recommendation: | Recommendation: | ||
* New competent cells need to be made from the Top10 as we need at least a transformation efficiency of 1 x 10^8 CFU/µg. | * New competent cells need to be made from the Top10 as we need at least a transformation efficiency of 1 x 10^8 CFU/µg. | ||
Latest revision as of 09:41, 4 September 2012
Competent Cell Testing
Results:
- The transformation efficiency for all of the Top10 cells were 1 x 10^7 CFU/µg while the transofmration efficiency for the Fusion Blue and BL21 cells were 1 x 10^6 CFU/µg.
Recommendation:
- New competent cells need to be made from the Top10 as we need at least a transformation efficiency of 1 x 10^8 CFU/µg.
