Team:TU Darmstadt/Protocols

From 2012.igem.org

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<span style="font-size:200%;"><span style="color:#00689D;">Protocols</span></span>
<span style="font-size:200%;"><span style="color:#00689D;">Protocols</span></span>
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== Protocols ==
The following page(s) gives an overview of the protocols and possible modifications that where used and proven working during the iGEM PET.erminators project 2012.
The following page(s) gives an overview of the protocols and possible modifications that where used and proven working during the iGEM PET.erminators project 2012.
== In vivo ==
== In vivo ==
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=== [[Escherichia coli]] ===
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=== [http://en.wikipedia.org/wiki/E._coli Escherichia coli] ===
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* [[Bacterial cell culture]]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Bacterial_cell_culture Bacterial cell culture]
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* [[Bacterial transformation]] aka TRAFO
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Bacterial_transformation Bacterial transformation] aka TRAFO
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* [[Cell counting/plating]]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Cell_counting/plating Cell counting/plating]
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* [[Chemically competent cells]]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Chemically_competent_cells Chemically competent cells]
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* [[Colony PCR]]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Colony_PCR Colony PCR]
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* [[Electrocompetent cells]]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Electrocompetent_cells Electrocompetent cells]
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* [[Electroporation]]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Electroporation Electroporation]
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* [[Engineering BioBrick vectors from BioBrick parts/Colony PCR]]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Engineering_BioBrick_vectors_from_BioBrick_parts/Colony_PCR Engineering BioBrick vectors from BioBrick parts/Colony PCR]
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* [[In-fusion BioBrick assembly]]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/In-fusion_BioBrick_assembly In-fusion BioBrick assembly]
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* [[Miniprep / Qiagen kit]]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Miniprep_/_Qiagen_kit Miniprep / Qiagen kit]
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* [[Transformation of chemically competent cells]]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Transformation_of_chemically_competent_cells Transformation of chemically competent cells]
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* [[Western Blot]]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Western_Blot Western blot]
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=== [[Comamonas testosteroni]] ===
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=== [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Comamonas_testosteroni Comamonas testosteroni] ===
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* [[Reviving a freez dried cell culture]]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Reviving_a_freez_dried_cell_culture Reviving a freez dried cell culture]
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== In vitro ==
== In vitro ==
===Nucleic acids ===
===Nucleic acids ===
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* [[Agarose gel electrophoresis]]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Agarose_gel_electrophoresis Agarose gel electrophoresis]
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* [[PCR techniques]]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/PCR_techniques PCR techniques]
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* [[SOE-PCR]]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/SOE-PCR SOE-PCR]
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=== [[DNA]] ===
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=== [http://en.wikipedia.org/wiki/DNA DNA] ===
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* [[Agarose gel electrohporesis]]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Agarose_gel_electrohporesis Agarose gel electrohporesis]
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* [[Annealing and primer extension]]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Annealing_and_primer_extension Annealing and primer extension]
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* [[Annealing primers]]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Annealing_primers Annealing primers]
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* [[Assembly PCR]]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Assembly_PCR Assembly PCR]
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* [[Cloning]]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Cloning Cloning]
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* [[DNA Ligation]]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/DNA_Ligation DNA Ligation]
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* [[DNA Quantification / NanoDrop]]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/DNA_Quantification_/_NanoDrop DNA Quantification / NanoDrop]
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* [[DNA Synthesis from Oligos]]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/DNA_Synthesis_from_Oligos DNA Synthesis from Oligos]
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* [[Engineering BioBrick vectors from BioBrick parts/Colony PCR]]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Engineering_BioBrick_vectors_from_BioBrick_parts/Colony_PCR Engineering BioBrick vectors from BioBrick parts/Colony PCR]
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* [[Miniprep]]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Miniprep Miniprep]
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* [[PCR]]
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* [http://en.wikipedia.org/wiki/Polymerase_chain_reaction PCR]
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* [[Polyacrylamide gel electrophoresis]]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Polyacrylamide_gel_electrophoresis Polyacrylamide gel electrophoresis]
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* [[Primer Design]]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Primer_Design Primer Design]
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* [[Purification of DNA]]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Purification_of_DNA  Purification of DNA]
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* [[Restriction digest]]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Restriction_digest Restriction digest]
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* [[Resuspension of primers]]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Resuspension_of_primers Resuspension of primers]
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* [[Salting Out]]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Salting_Out Salting Out]
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* [[Sequencing DNA]]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Sequencing_DNA Sequencing DNA]
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=== [[RNA]] ===
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=== [http://en.wikipedia.org/wiki/RNA RNA] ===
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=== [[Protein]] ===
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=== [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Protein Protein] ===
== In silico ==
== In silico ==
=== Data analysis ===
=== Data analysis ===
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* [[R Programming]]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/R_Programming R Programming]
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* [[Mathlab / Simulink]]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Mathlab_/_Simulink Mathlab / Simulink]
=== Databases ===
=== Databases ===
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* [[PDB]]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/PDB PDB]
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* [[CAS]]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/CAS CAS]
=== Modelling ===
=== Modelling ===
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* [[SVEN!!!]]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Modelling]
=== Sequence analysis ===
=== Sequence analysis ===
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* [[BLAST]]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/BLAST BLAST]
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* [[SAGE]]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/SAGE SAGE]
=== Structure analysis ===
=== Structure analysis ===
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* [[PDB]]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/PDB PDB]
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* [[Protein structure prediction]]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Protein_structure_prediction Protein structure prediction]
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* [[RNA secondary structure prediction]]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/RNA_secondary_structure_prediction RNA secondary structure prediction]
== Miscellaneous ==
== Miscellaneous ==
=== Cell culture ===
=== Cell culture ===
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* [[Pouring LB plates]]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Pouring_LB_plates Pouring LB plates]
=== Microscopy ===
=== Microscopy ===
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* [[Determining an objects size]]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Determining_an_objects_size Determining an objects size]
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* [[Preparing slides]]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Preparing_slides Preparing slides]
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* [[Staining]]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Staining Staining]
=== ILDs / Improvised Lab Devices ===
=== ILDs / Improvised Lab Devices ===
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* [[x bp Primer Designer]]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/x_bp_Primer_Designer x bp Primer Designer]
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* [[Enhancing a thermo cyclers cooling capabilites]]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Enhancing_a_thermo_cyclers_cooling_capabilites Enhancing a thermo cyclers cooling capabilites]
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* [[Gel pocket size reduction]]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Gel_pocket_size_reduction Gel pocket size reduction]
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* [[Growing iGEMlers]]
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Growing_iGEMlers Growing iGEMlers]

Revision as of 04:10, 6 September 2012

Protocols

Contents

Protocols

The following page(s) gives an overview of the protocols and possible modifications that where used and proven working during the iGEM PET.erminators project 2012.

In vivo

[http://en.wikipedia.org/wiki/E._coli Escherichia coli]

Comamonas testosteroni

In vitro

Nucleic acids

[http://en.wikipedia.org/wiki/DNA DNA]

[http://en.wikipedia.org/wiki/RNA RNA]

Protein

In silico

Data analysis

Databases

Modelling

Sequence analysis

Structure analysis

Miscellaneous

Cell culture

Microscopy

ILDs / Improvised Lab Devices

Retrieved from "http://2012.igem.org/Team:TU_Darmstadt/Protocols"