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Team:NRP-UEA-Norwich/Week9
From 2012.igem.org
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==Day 1== | ==Day 1== | ||
| - | . We looked at the plates of transformant colonies of RFP and GFP that have kanamycin resistance. Both Rebecca's and Rachel's plates for GFP looked a little dodgy. Rebecca's had not grown at all whereas, Rachel's had | + | . We looked at the plates of transformant colonies of RFP and GFP that have kanamycin resistance. Both Rebecca's and Rachel's plates for GFP looked a little dodgy. Rebecca's had not grown at all whereas, Rachel's had but the colonies were rather large. The RFP colonies looked good. However, after double checking in the parts registry, we realised that these BioBricks have no RBS and therefore, we decided to keep these plates for now and went back to the previous BioBricks used with RFP, GFP and CFP. |
. Russell, Rebecca and Khadija used ''Pst''1 and ''Xba''1 to do a double restriction digest of RFP, GFP and CFP that we previously used. We decided that despite the backbone fragment seemingly being too large, we would try it out. | . Russell, Rebecca and Khadija used ''Pst''1 and ''Xba''1 to do a double restriction digest of RFP, GFP and CFP that we previously used. We decided that despite the backbone fragment seemingly being too large, we would try it out. | ||
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| + | . At the same time, as we were using isolated plasmid DNA we decided to transform the original DNA into alpha competent silver standard cells from Bioline. | ||
==Day 2== | ==Day 2== | ||
Revision as of 17:53, 3 September 2012
