Team:Peking

From 2012.igem.org

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<p>During the last few decades, chemically regulated genetic systems have been thoroughly developed and analyzed. Though remarkable endeavors were made towards this issue, the disadvantages of chemical regulation remain: the high cost of chemical synthesis, the diffusion limits, the insecurity and the limited choices of chemicals. <br /><br /> Light, in contrast, is more controllable to regulate molecular and cellular behavior. However, most optogenetics methods rely on laser, which limits their field application. <br /><br /> This summer, Peking iGEM is endeavoring on developing a new ultra-sensitive luminescence sensor and has programmed cells to talk through light. </p>
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<p>Optogenetic tools have made significant impact on life sciences and beyond. However, several serious issues remain:
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cytotoxicy, narrow dynamic range, and dependency on laser and exogenous chromophore. To circumvent these, Peking
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iGEM has rationally constructed a hypersensitive sensor of luminance- Luminesensor. Primarily, the sensor was designed
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by fusing blue-light-sensing protein domain from Neurospora with DNA binding domain of LexA from E.coli, following which
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protein structure inspection and kinetic simulation were conducted to rationally perform optimization. Amazingly,
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Luminesensor was proved to be as sensitive as to sense natural light and even bioluminescence. With this sensor,
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spatiotemporal control of cellular behavior, such as phototaxis, high-resolution 2-D and 3-D bio-printing using dim light and
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even luminescence of iPad were shown to be very easy. What’s more, we successfully implemented cell-cell signaling
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using light, which is the very first time in synthetic biology and of great importance for biotechnological use.</p>
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<p>Project Quickview and Wiki Page is comming soon...</p>
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<p>/*During the last few decades, chemically regulated genetic systems have been thoroughly developed and analyzed. Though remarkable endeavors were made towards this issue, the disadvantages of chemical regulation remain: the high cost of chemical synthesis, the diffusion limits, the insecurity and the limited choices of chemicals. <br /><br /> Light, in contrast, is more controllable to regulate molecular and cellular behavior. However, most optogenetics methods rely on laser, which limits their field application. <br /><br /> This summer, Peking iGEM is endeavoring on developing a new ultra-sensitive luminescence sensor and has programmed cells to talk through light.*/ </p>
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Revision as of 10:52, 3 September 2012

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