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Team:KAIT Japan/Notebook
From 2012.igem.org
(Difference between revisions)
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=Creating parts of Tar methylation region= | =Creating parts of Tar methylation region= | ||
==Date:8/9== | ==Date:8/9== | ||
| - | + | Colony PCR | |
::'''→Reflection:We Took E.coli too many.You should take less E.coli.'''<br> | ::'''→Reflection:We Took E.coli too many.You should take less E.coli.'''<br> | ||
::'''→Reflection:The number of cycles was less.So,We increased The number of cycles in 8/11.(50 cycles) ''' | ::'''→Reflection:The number of cycles was less.So,We increased The number of cycles in 8/11.(50 cycles) ''' | ||
| Line 61: | Line 61: | ||
==Date:8/11== | ==Date:8/11== | ||
| - | + | The purified DNA | |
| - | + | ::'''→Reflection:Band was less.''' | |
===PCR Product=== | ===PCR Product=== | ||
| Line 69: | Line 69: | ||
==Date:8/13== | ==Date:8/13== | ||
| - | + | Confirmed of electrophoresis by PCR product and Ligation of the TA vector | |
---- | ---- | ||
==Date:8/14== | ==Date:8/14== | ||
| - | + | Transformation | |
---- | ---- | ||
==Date:8/25== | ==Date:8/25== | ||
| - | + | Miniprep | |
=Creating parts of azurin= | =Creating parts of azurin= | ||
==Date:8/16== | ==Date:8/16== | ||
| - | + | Colony PCR | |
| - | + | Ligation | |
---- | ---- | ||
==Date:8/18== | ==Date:8/18== | ||
| - | + | Colony PCR | |
---- | ---- | ||
==Date:8/20== | ==Date:8/20== | ||
| - | + | DNA extraction and purification of ''P.aeruginosa'' | |
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Revision as of 16:39, 2 September 2012
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Creating parts of Tar methylation regionDate:8/9Colony PCR
Date:8/11The purified DNA
PCR ProductDate:8/13Confirmed of electrophoresis by PCR product and Ligation of the TA vector Date:8/14Transformation Date:8/25Miniprep Creating parts of azurinDate:8/16Colony PCR Ligation Date:8/18Colony PCR Date:8/20DNA extraction and purification of P.aeruginosa |
