Team:Macquarie Australia/Protocols/ArrivalofGBlocks

From 2012.igem.org

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(→Ordered gBlock fragments are nearly here!)
(→Ordered gBlock fragments are nearly here!)
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!
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! Heme oxygenase
+
! Heme oxygenase (T7 minus)
! Plasmid
! Plasmid
! Hemo_A
! Hemo_A
! Hemo_B
! Hemo_B
 +
!
 +
!
 +
!
|-
|-
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! T7 Agro
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! Plasmid
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! Agro_T7_A
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! Agro_B
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! Agro_C
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! Agro_D
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! Agro_E
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|-
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! Agro (T7 minus)
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! Plasmid
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! Agro_A
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! Agro_B
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! Agro_C
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! Agro_D
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! Agro_E

Revision as of 05:17, 31 August 2012



Ordered gBlock fragments are nearly here!

Soon our gBlock fragments will arrive from IDT. On Tuesday (4/09/2012) we are going to perform Gibson assembly will assemble the following four biobricks:

  • +T7 promoter Agrobacterium tumefaciens bacteriophytochrome
  • -T7 promoter Agrobacterium tumefaciens bacteriophytochrome
  • +T7 promoter Heme oxygenase
  • -T7 promoter Heme oxygenase

Click on each above to see fragments which will be assembled using Gibson and fragment properties.


Biobrick Name Part(s)
T7 Heme oxygenase Plasmid Hemo_T7_A Hemo_B
Heme oxygenase (T7 minus) Plasmid Hemo_A Hemo_B
T7 Agro Plasmid Agro_T7_A Agro_B Agro_C Agro_D Agro_E
Agro (T7 minus) Plasmid Agro_A Agro_B Agro_C Agro_D Agro_E
Retrieved from "http://2012.igem.org/Team:Macquarie_Australia/Protocols/ArrivalofGBlocks"