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Team:Macquarie Australia/Protocols/ArrivalofGBlocks
From 2012.igem.org
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Soon our gBlock fragments will arrive from IDT. On Tuesday (4/09/2012) we are going to perform Gibson assembly will assemble the following four biobricks: | Soon our gBlock fragments will arrive from IDT. On Tuesday (4/09/2012) we are going to perform Gibson assembly will assemble the following four biobricks: | ||
| - | * +T7 promoter Agrobacterium tumefaciens | + | * +T7 promoter Agrobacterium tumefaciens bacteriophytochrome |
| - | * -T7 promoter Agrobacterium tumefaciens | + | * -T7 promoter Agrobacterium tumefaciens bacteriophytochrome |
| - | * +T7 promoter Heme oxygenase | + | * +T7 promoter Heme oxygenase |
* -T7 promoter Heme oxygenase | * -T7 promoter Heme oxygenase | ||
| + | |||
| + | Click on each above to see fragments which will be assembled using Gibson and fragment properties. | ||
| + | |||
| + | |||
| + | BioBrick name Part(s) | ||
| + | T7 Heme oxygenase Plasmid Hemo_T7_A Hemo_B | ||
| + | Heme oxygenase Plasmid Hemo_A Hemo_B | ||
| + | T7 Agro Plasmid Agro_T7_A Agro_B Agro_C Agro_D Agro_E | ||
| + | Agro Plasmid Agro_A Agro_B Agro_C Agro_D Agro_E | ||
Revision as of 05:12, 31 August 2012
Ordered gBlock fragments are nearly here!
Soon our gBlock fragments will arrive from IDT. On Tuesday (4/09/2012) we are going to perform Gibson assembly will assemble the following four biobricks:
- +T7 promoter Agrobacterium tumefaciens bacteriophytochrome
- -T7 promoter Agrobacterium tumefaciens bacteriophytochrome
- +T7 promoter Heme oxygenase
- -T7 promoter Heme oxygenase
Click on each above to see fragments which will be assembled using Gibson and fragment properties.
BioBrick name Part(s)
T7 Heme oxygenase Plasmid Hemo_T7_A Hemo_B
Heme oxygenase Plasmid Hemo_A Hemo_B
T7 Agro Plasmid Agro_T7_A Agro_B Agro_C Agro_D Agro_E
Agro Plasmid Agro_A Agro_B Agro_C Agro_D Agro_E
