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Team:Paris Bettencourt/Semantic containment
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<div id="grouptitle">Semantic containment</div> | <div id="grouptitle">Semantic containment</div> | ||
==Overview== | ==Overview== | ||
| - | + | We need to prevent our genetic construction from being used by other organism. Since horizontal gene transfer (HGT) can be perform either by conjugation, by transduction, or by transformation, and none of these systems is only dependent of our organism, we cannot assume the fact that HGT is fully avoidable. Semantic containment [1] means that our bacteria won't be able to "speak" with other organism, since they don't speak the same language. The language being DNA. Here, it won't be all DNA that we are going to change, but just 1 stop codon that we are going to change in a normal amino-acid codon in our synthetic bacteria. It means that in our bacteria the stop codon will be translate into an amino-acids, whereas in wild type bacteria, the gene won't be translate, and the protein won't be efficient. | |
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==Objectives== | ==Objectives== | ||
Revision as of 17:05, 2 September 2012
- Main
- Team
- Project
- Achievements
- Human Practice
- Safety
- Notebook
- Attributions
Overview
Delay system
Semantic containment
Restriction enzyme system
MAGE
Suicide system
Encapsulation
Synthetic import domain
Safety Questions
Safety AssessmentContents |
Overview
We need to prevent our genetic construction from being used by other organism. Since horizontal gene transfer (HGT) can be perform either by conjugation, by transduction, or by transformation, and none of these systems is only dependent of our organism, we cannot assume the fact that HGT is fully avoidable. Semantic containment [1] means that our bacteria won't be able to "speak" with other organism, since they don't speak the same language. The language being DNA. Here, it won't be all DNA that we are going to change, but just 1 stop codon that we are going to change in a normal amino-acid codon in our synthetic bacteria. It means that in our bacteria the stop codon will be translate into an amino-acids, whereas in wild type bacteria, the gene won't be translate, and the protein won't be efficient.
Objectives
Write here the objectives of your project
Design
Present the design of your system, both in a written form, and a schematic one.
Experiments and results
Characterisation of X
Experimental setup
Describe the experiment
Results
Present your results
Testing of the system
Experimental setup
Describe the experiment
Results
Present your results
