Team:Potsdam Bioware

From 2012.igem.org

(Difference between revisions)
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<div class="box_round block">
<div class="box_round block">
===Team===
===Team===
-
 
+
  <div id="portfolio">
 +
      <div class="team_box">Mario Köhler</div>
 +
      <div class="team_box">Tom Scheidt</div>
 +
      <div class="team_box">Christopher Kressler</div>
 +
      <div class="team_box">Tobias Priemel</div>
 +
      <div class="team_box">Maria Kunert</div>
 +
      <div class="team_box">Sascha Lehnort</div>
 +
      <div class="team_box">Norman Hein</div>
 +
  </div>
</div>
</div>
Line 151: Line 159:
#p-logo img {
#p-logo img {
   box-shadow:5px 5px 5px #666;
   box-shadow:5px 5px 5px #666;
 +
}
 +
 +
.center_box {
 +
  text-align: center;
}
}
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*
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* Copyright © 2008 George McGinley Smith
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function(){
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);
-
/*
 
-
*
 
-
* TERMS OF USE - EASING EQUATIONS
 
-
*
 
-
* Open source under the BSD License.
 
-
*
 
-
* Copyright © 2001 Robert Penner
 
-
* All rights reserved.
 
-
*
 
-
* Redistribution and use in source and binary forms, with or without modification,
 
-
* are permitted provided that the following conditions are met:
 
-
*
 
-
* Redistributions of source code must retain the above copyright notice, this list of
 
-
* conditions and the following disclaimer.
 
-
* Redistributions in binary form must reproduce the above copyright notice, this list
 
-
* of conditions and the following disclaimer in the documentation and/or other materials
 
-
* provided with the distribution.
 
-
*
 
-
* Neither the name of the author nor the names of contributors may be used to endorse
 
-
* or promote products derived from this software without specific prior written permission.
 
-
*
 
-
* THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS "AS IS" AND ANY
 
-
* EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF
 
-
* MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL THE
 
-
*  COPYRIGHT OWNER OR CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL,
 
-
*  EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF SUBSTITUTE
 
-
*  GOODS OR SERVICES; LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED
 
-
* AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING
 
-
*  NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED
 
-
* OF THE POSSIBILITY OF SUCH DAMAGE.
 
-
*
 
-
*/
 
-
$('#s3').cycle({
 
-
    fx:    'fade',
 
-
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    timeout: 3000,
 
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-
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</script>
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</head>
</head>
</html>
</html>

Revision as of 12:32, 25 August 2012

Home Team Official Team Profile Project Parts Submitted to the Registry Modeling Notebook Safety Attributions



Direct Antibody Identification, Maturation and Production in CHO-Cells

Project Description

Antibodies are versatile molecules for research and therapy and they are currently revolutionizing the market of biopharmaceuticals. To date, antibodies are generated in a laborious and time consuming manner. Either animal immunization followed by hybridoma technology or phage display is used to identify desired genes, which then need to be transferred to a production cell line such as CHO. We designed a streamlined workflow that incorporates all steps of antibody generation in CHO cells. Our plan is to stably transfect an antibody-fragment library in CHO cells. Antibody maturation is mimicked by further diversifying this library using the enzyme Activation-Induced cytidine Deaminase (AID), which is known to induce somatic hypermutation. Next, we plan to test and deploy a versatile and continuous viral selection system for clones producing the desired antibodies. Finally, we will try to employ a genetic switch to go from surface expression to soluble production of antibodies.


Team

Mario Köhler
Tom Scheidt
Christopher Kressler
Tobias Priemel
Maria Kunert
Sascha Lehnort
Norman Hein


Retrieved from "http://2012.igem.org/Team:Potsdam_Bioware"