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Team:University College London/Module 3/Design
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'''Requirement 1: The bacteria should carry a system capable of degrading polythelene''' | '''Requirement 1: The bacteria should carry a system capable of degrading polythelene''' | ||
| - | Laccase, an oxidoreductive enzyme, is important in bioremediation. Laccase from Rhodococcus | + | Laccase, an oxidoreductive enzyme, is important in bioremediation. Laccase from ''Rhodococcus Ruber'' C208 has been found to be involved in polyethylene biodegradation (http://www.sciencedirect.com/science/article/pii/S0964830512000637). The Laccase mechanism involves the generation of free radicals which encourages degradation of polyethylene. As Rhodococcus strains are presently unavailable to us, our system includes cueO, a laccase/multi-copper oxidase from E. coli. |
'''Requirement 2: The system must be compatible with the marine environment''' | '''Requirement 2: The system must be compatible with the marine environment''' | ||
| - | Our chosen enzyme has been suggested to survive in harsh conditions; | + | Our chosen enzyme has been suggested to survive in harsh conditions; Laccase is thermostable (http://www.ncbi.nlm.nih.gov/pubmed/14586105) and can function within a broad pH range, (http://www.sciencedirect.com/science/article/pii/S0960852412002234) therefore we expect our system will function in the marine environment. |
{{:Team:University_College_London/templates/foot}} | {{:Team:University_College_London/templates/foot}} | ||
Revision as of 09:56, 24 August 2012
Module 3: Design
Description | Design | Construction | Characterisation | Modelling | Results | Conclusions
Description
We identified two requirements for this system, and have designed the module to meet each requirement.
Requirement 1: The bacteria should carry a system capable of degrading polythelene
Laccase, an oxidoreductive enzyme, is important in bioremediation. Laccase from Rhodococcus Ruber C208 has been found to be involved in polyethylene biodegradation (http://www.sciencedirect.com/science/article/pii/S0964830512000637). The Laccase mechanism involves the generation of free radicals which encourages degradation of polyethylene. As Rhodococcus strains are presently unavailable to us, our system includes cueO, a laccase/multi-copper oxidase from E. coli.
Requirement 2: The system must be compatible with the marine environment
Our chosen enzyme has been suggested to survive in harsh conditions; Laccase is thermostable (http://www.ncbi.nlm.nih.gov/pubmed/14586105) and can function within a broad pH range, (http://www.sciencedirect.com/science/article/pii/S0960852412002234) therefore we expect our system will function in the marine environment.
