Team:NRP-UEA-Norwich/Week6

From 2012.igem.org

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==Day 1==
==Day 1==
-
. We have BioBricks!
+
===Labs===
-
- Practice run of growth study with absorbance readings only. Contamination. Lasted 6 hours.
+
. We have BioBricks!  
 +
 
 +
. Practice run of growth study with absorbance readings only. Contamination. Lasted 6 hours.
==Day 2==
==Day 2==
-
- Rebecca did 9 hour growth study with wavescan function
+
===Labs===
-
-Over 50 plates to generate more cells.
+
. LB media and Agar media was made in vast quantities.
 +
 
 +
.  Rebecca did a 9 hour growth study with PyeaR transfoormed cells and Bioline alpha ''E.coli'' cells as a comparison. This was done with wavescan function and single wavelength absorbance at 660nm which is not absorbed by pure LB. Recordings were taken every hour and a serial dilution was made and the two lowest dilutions were plated (10^-4 and 10^-5).  
==Day 3==
==Day 3==
-
- Forum preparation - plates with Biobrick written with one letter per plate (using the pYEAR with GFP and potassium nitrate to induce promoter activity)
+
===Labs===
-
- Rebecca, Khadija and Luas, used day 2 samples and carried out a growth study with a reading every minute for 1 hour.
+
. Forum preparation - plates with Biobrick written with one letter per plate (using the pYEAR with GFP and potassium nitrate to induce promoter activity)
 +
 
 +
. Rebecca, Khadija and Lucas, used day 2 samples and carried out a growth study with a reading every minute for 1 hour.
==Day 4==
==Day 4==

Revision as of 20:31, 23 August 2012

Header1NewGreen.png

NRP UEA iGEM 2012

 

Welcome to the NRP UEA iGEM 2012 Wiki Lab Book

Please choose the relevant link to access our diary of that week!

Week Zero | Week One | Week Two | Week Three | Week Four | Week Five | Week Six | Week Seven | Week Eight | Week Nine | Week Ten | Week Eleven | Lab Protocols | Experiments

Contents

Week 6

Day 1

Labs

. We have BioBricks!

. Practice run of growth study with absorbance readings only. Contamination. Lasted 6 hours.

Day 2

Labs

. LB media and Agar media was made in vast quantities.

. Rebecca did a 9 hour growth study with PyeaR transfoormed cells and Bioline alpha E.coli cells as a comparison. This was done with wavescan function and single wavelength absorbance at 660nm which is not absorbed by pure LB. Recordings were taken every hour and a serial dilution was made and the two lowest dilutions were plated (10^-4 and 10^-5).

Day 3

Labs

. Forum preparation - plates with Biobrick written with one letter per plate (using the pYEAR with GFP and potassium nitrate to induce promoter activity)

. Rebecca, Khadija and Lucas, used day 2 samples and carried out a growth study with a reading every minute for 1 hour.

Day 4

- Forum preparation - plates used for the forum science day had their fluorescence intensified by adding more potassium nitrate to the cells on the plate.

Day 5

. UK Team Meet-Up!

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