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Team:Bielefeld-Germany/Labjournal/week17
From 2012.igem.org
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===Tuesday August 21st=== | ===Tuesday August 21st=== | ||
===Wednesday August 22nd=== | ===Wednesday August 22nd=== | ||
| - | '''Street Science''' | + | '''Street Science:''' |
Went to Dr. Joe Max Risse today and asked him about the mircoorganisms of the Fermentationgroup. He recommended ''Euglena gracilis'', since it is without risk, big, colorful, and very healthy under the microscop. | Went to Dr. Joe Max Risse today and asked him about the mircoorganisms of the Fermentationgroup. He recommended ''Euglena gracilis'', since it is without risk, big, colorful, and very healthy under the microscop. | ||
I asked for the bacteria they have and he told me I could check if their ''Kocuria rosea'' is a wild type. | I asked for the bacteria they have and he told me I could check if their ''Kocuria rosea'' is a wild type. | ||
In the DMSZ catalog there are three strains of ''Kocuria rosea'' and all have been assessed to be of riskgroup 1, which is the safest group of bacteria and means it is unlikely that it will infect humans. | In the DMSZ catalog there are three strains of ''Kocuria rosea'' and all have been assessed to be of riskgroup 1, which is the safest group of bacteria and means it is unlikely that it will infect humans. | ||
I also asked for ''Penicillium chrysogenum'' but the one they use is a GVO. | I also asked for ''Penicillium chrysogenum'' but the one they use is a GVO. | ||
| + | * '''Team Cellulose Binding Domain:''' | ||
| + | Test-restriktion of all isolated CBDcex- and the GFP_Freiburg-plasmids with NotI - no positiv result | ||
| + | Started new and made a gradient PCR for GFP-Freiburg and CBDcex (50-70°C) | ||
===Thursday August 23rd=== | ===Thursday August 23rd=== | ||
Revision as of 17:00, 22 August 2012
Contents |
Labjournal
Week1 Week2 Week3 Week4 Week5 Week6 Week7 Week8 Week9 Week10 Week11 Week12 Week13 Week14 Week15 Week16 Week17 Week18
Week 17 (08/20 - 08/26/12)
Monday August 20th
Tuesday August 21st
Wednesday August 22nd
Street Science: Went to Dr. Joe Max Risse today and asked him about the mircoorganisms of the Fermentationgroup. He recommended Euglena gracilis, since it is without risk, big, colorful, and very healthy under the microscop. I asked for the bacteria they have and he told me I could check if their Kocuria rosea is a wild type. In the DMSZ catalog there are three strains of Kocuria rosea and all have been assessed to be of riskgroup 1, which is the safest group of bacteria and means it is unlikely that it will infect humans. I also asked for Penicillium chrysogenum but the one they use is a GVO.
- Team Cellulose Binding Domain:
Test-restriktion of all isolated CBDcex- and the GFP_Freiburg-plasmids with NotI - no positiv result Started new and made a gradient PCR for GFP-Freiburg and CBDcex (50-70°C)
