Team:Cambridge/Protocols
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* [[Team:Cambridge/Protocols/SDSPAGE|<u><span style="color:#00000CD">SDS PAGE protein analysis</span></u>]] A method used to separate polypeptides of different lengths. | * [[Team:Cambridge/Protocols/SDSPAGE|<u><span style="color:#00000CD">SDS PAGE protein analysis</span></u>]] A method used to separate polypeptides of different lengths. | ||
* [[Team:Cambridge/Protocols/RestrictionDigest|<u><span style="color:#00000CD">Restriction Enzyme Digest</span></u>]] A method for creating a restriction map of a plasmid. | * [[Team:Cambridge/Protocols/RestrictionDigest|<u><span style="color:#00000CD">Restriction Enzyme Digest</span></u>]] A method for creating a restriction map of a plasmid. | ||
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* [[Team:Cambridge/Protocols/TransformationofB.subtilis|<u><span style="color:#00000CD">Transformation of ''Bacillus subtilis''</span></u>]] A technique used to introduce foreign DNA into competent Bacillus cells. | * [[Team:Cambridge/Protocols/TransformationofB.subtilis|<u><span style="color:#00000CD">Transformation of ''Bacillus subtilis''</span></u>]] A technique used to introduce foreign DNA into competent Bacillus cells. | ||
* [[Team:Cambridge/Protocols/TransformationofE.coli|<u><span style="color:#00000CD">Transformation of ''Escherichia coli''</span></u> ]] A method for transforming competent ''E.coli'' with DNA | * [[Team:Cambridge/Protocols/TransformationofE.coli|<u><span style="color:#00000CD">Transformation of ''Escherichia coli''</span></u> ]] A method for transforming competent ''E.coli'' with DNA | ||
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+ | ==Recipes== | ||
+ | * [[Team:Cambridge/Protocols/TAEBuffer|<u><span style="color:#00000CD">TAE buffer for gel electrophoresis</span></u>]] A recipe for making 10xTAE buffer | ||
[[Team:Cambridge/Safety/RiskAssessments|Associated risk assessments]] | [[Team:Cambridge/Safety/RiskAssessments|Associated risk assessments]] |
Revision as of 18:17, 23 August 2012
Protocols
- β-galactosidase assay Assay to measure the amount of the enzyme β-galactosidase being produced by a population of cells. Useful as a reporter system.
- BioBricks Resources for manipulation of BioBricks and information regarding the distribution.
- Colony PCR PCR with cells as a template. Useful for checking the length of an insert in an introduced plasmid.
- Chemically competent cells generation A technique to produce e.coli cellsreceptive to chemical transformation.
- Electocompetent cells generation A technique to produce e.coli cells receptive to transformation by electroporation
- Electroporation A method for transforming appropriately competent cells with plasmid DNA using electricity.
- Gel Electrophoresis A technique for separating DNA strands of different lengths.
- Gel Extraction of DNA A technique used to isolate a desired fragment of intact DNA from an agarose gel following agarose gel electrophoresis.
- Gibson Assembly A technique for ligating multiple DNA fragments in one step, compatible with standard assembly.
- Glycerol stocks A technique for long term storage of cells at -80 degrees without losing cell vitality.
- IPTG induction A technique for inducing the pSPANK promoter in E.coli cells.
- LB Agar Plates preparation A method used to prepare agar plate to culture common bacteria.
- MiniPrep - DNA extraction A method used to extract DNA from bacterial cells.
- PCR using a high temperature DNA polymerase A method for amplifying a section of DNA.
- SDS PAGE protein analysis A method used to separate polypeptides of different lengths.
- Restriction Enzyme Digest A method for creating a restriction map of a plasmid.
- Transformation of Bacillus subtilis A technique used to introduce foreign DNA into competent Bacillus cells.
- Transformation of Escherichia coli A method for transforming competent E.coli with DNA
Recipes
- TAE buffer for gel electrophoresis A recipe for making 10xTAE buffer
Lab supplies:
- [http://www.bioc.cam.ac.uk/stores/ BioPath Stores]
- [http://www.plantsci.cam.ac.uk/camonly/stores/stock.xls Plant Department Stores Catalogue xls (Updated 2nd July 2012)]