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Team:Lyon-INSA/notebook
From 2012.igem.org
(Difference between revisions)
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</jour> | </jour> | ||
<jour nb="2"> | <jour nb="2"> | ||
| - | <titre> | + | <titre>For all purposes</titre> |
| - | <date> 2nd | + | <date> Monday, July 2nd 2012</date> |
| - | + | ||
<description>Liquid culture (5 mL LB media) of NM 522 cells and overnight incubation under agitation at 37°C for later transformations.</description> | <description>Liquid culture (5 mL LB media) of NM 522 cells and overnight incubation under agitation at 37°C for later transformations.</description> | ||
<diaporama></diaporama> | <diaporama></diaporama> | ||
| - | </jour> | + | </jour> |
| + | <jour nb="3"> | ||
| + | <titre>For all purposes</titre> | ||
| + | <date> Tuesday, July 3rd 2012</date> | ||
| + | <description>Dilution of 100 µL saturated culture in 5 mL LB media. | ||
| + | Incubation time : 2 hours (until O.D =0,3). | ||
| + | Transformation of the NM 522 strain (this experiment was made 3 times) | ||
| + | |||
| + | For the positive control the pSB1C3 plasmid was used ; | ||
| + | For the transformation, the pBBA_I742123 was used (well A2, 5th iGEM kit plate) | ||
| + | The transformed bacteria were selected on chloramphenicol plates. | ||
| + | </description> | ||
| + | <diaporama></diaporama> | ||
| + | </jour> | ||
| + | |||
</month> | </month> | ||
</project> | </project> | ||
Revision as of 21:52, 20 August 2012
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