Team:Osaka/week2

(Difference between revisions)

August 5 (Sun)

1. Miniprep of yesterday's culture
2. Restriction digests of minipreppped parts
3. Gel electrophoresis of digests
4. Transfer to liquid culture
   • (Rosetta)143~146,149,152
   • (DH5α)150,Empty vector(A)

August 6 (Mon)

1. Miniprep of yesterday's culture
2. Restriction digests of minipreppped parts
3. Gel electrophoresis of digests
4. Transformation of Registry parts into DH5α E.coli(See Table 2)
5. Preparation of glycerol stock of cell culture:143~146,149,152

Table2

ID	Part Name	Resistance	Description
1-2D	<bbpart>BBa_J52008</bbpart>	A,K	Renilla Luciferase
1-10H	<bbpart>BBa_I712019</bbpart>	A,K	Firefly Luciferase from Photinus pyralis
1-22A	<bbpart>BBa_J23118</bbpart>	A	Constitutive promoter family member

August 7 (Tue)

1. Preparation of LB agar plates (Cam)
2. Transformation of 1-2D,1-10H

August 8 (Wed)

1. Transfer to liquid culture:1-2D,1-10H,1-22A,151

August 9 (Thu)

1. Miniprep of yesterday's culture
2. Restriction digests of minipreppped parts
3. Gel electrophoresis of digests
   • 1-10H not ok
4. Transformaton of Registry parts into DH5α E.coli(See Table 3)
5. Transformation of plasmid DNA into Rosetta E.coli:147,148,150

Table3

ID	Part Name	Resistance	Description
1-2M	<bbpart>BBa_K118022</bbpart>	A	RBS

August 10 (Fri)

1. Sequencing reaction:143~152
2. Sequencing purification
3. Transfer to liquid culture:1-2M,147,148,150
4. Transformation of Registry parts into DH5α E.coli:1-10H

August 11 (Sat)

1. Preparation of glycerol stock of cell culture:147,148,150
2. Miniprep of yesterday's culture:1-2M
3. Restriction digests of minipreppped parts
4. Transfer to liquid culture:1-10H
5. SDS-PAGE :149,150,151,152,empty vector
   • IPTG induction

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