Team:Wageningen UR/Journal/week8

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*6 different Coat Protein (CP) constructs were PCR amplified from the TuYV genome, adding iGEM pre- and suffixes and a Histidine tag.
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*With designed primers, six different constructs were isolated and amplified from TuYV genome, also iGEM prefix and suffix were added to each construct. The six constructs are coat protein of TuYV, coat protein of TuYV with His-tag, Coat protein + 1/2 readthrough, Coat protein + 1/2 readthrough with His-tag, coat protein + readthrough, coat protein + readthroug, later they will be named 1,1H,2,2H,3,3H.  
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Written by: Wouter
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Written by: Han Yue
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[[https://2012.igem.org/Team:Wageningen_UR/Journal/week7 previous week]]          [[https://2012.igem.org/Team:Wageningen_UR/Journal/week9 next week]]
[[https://2012.igem.org/Team:Wageningen_UR/Journal/week7 previous week]]          [[https://2012.igem.org/Team:Wageningen_UR/Journal/week9 next week]]

Revision as of 05:01, 18 September 2012

week 8: 18 june - 24 june

The DNA of our Polero virus finally arrived! It turns out not to be the Potato Leafroll Virus (PLRV), but instead the Turnip Yellows Virus (TuYV) which is a close relative (also from the Polero genus).

Office work

Lab work

TuYV

Friday:

  • With designed primers, six different constructs were isolated and amplified from TuYV genome, also iGEM prefix and suffix were added to each construct. The six constructs are coat protein of TuYV, coat protein of TuYV with His-tag, Coat protein + 1/2 readthrough, Coat protein + 1/2 readthrough with His-tag, coat protein + readthrough, coat protein + readthroug, later they will be named 1,1H,2,2H,3,3H.


Written by: Han Yue

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