Team:Osaka/week3

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# Miniprep of yesterday's culture
# Miniprep of yesterday's culture
# PCR
# PCR
 +
#*mutation was found in PpA →　retrial
# Restriction digests of minipreppped parts
# Restriction digests of minipreppped parts
# Gel electrophoresis of digests  
# Gel electrophoresis of digests  

Revision as of 05:52, 18 August 2012

August 12 (Sun)

  1. Miniprep of yesterday's culture
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests
  4. Ligation
    • 153(C):1-2M(upstream)+1-10H(downstream)+1-1A(vector)
    • 154(C):1-22A(upstream)+1-2M(downstream)+1-1A(vector)
    • 155(C):1-2D(upstream)+1-23L(downstream)+1-1A(vector)
  5. SDS-PAGE
    • Gel electrophoresis

August 16 (Thu)

  1. Transformaton of ligation products into DH5α E.coli:153~155
  2. Transformation of plasmid DNA into Rosetta E.coli:empty vector(A)

August 17 (Fri)

  1. Transfer to liquid culture

August 18 (Sat)

  1. Preparation of LB agar plates
  2. Miniprep of yesterday's culture
  3. PCR
    • mutation was found in PpA →　retrial
  4. Restriction digests of minipreppped parts
  5. Gel electrophoresis of digests
  6. PCR purification
  7. Ligation

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