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Team:UC Chile2/Cyanolux/Constructs
From 2012.igem.org
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| + | <p><font face="Calibri"><font size="3">C3</font><br> | ||
| + | Construct designed from pPMQAK1 expression plasmid, which replicates both in E. coli and cyanobacteria and is biobrick-compatible. It codes for RFP under synechocystis psbAB promoter and cuts out the whole Ccdb toxin gene. | ||
| + | </font></p> | ||
Revision as of 22:39, 15 August 2012
C1.1
Integrative plasmid designed for the expression of RFP under synechocystis psbAB promoter also with a kanamycin resistance cassette for selection in cyanobacteria. The construct is flanked by neutral recombination sites RS1 and RS2, homologous to synechocystis chromosome.
C1.2
This integrative plasmid is designed for the expression of RFP under synechocystis psaA2 promoter with a kanamycin resistance cassette for selection in cyanobacteria. The construct is flanked by neutral recombination sites RS1 and RS2, homologous to synechocystis chromosome.
C2.1
Construct designed from pPMQAK1 expression plasmid, which replicates both in E. coli and cyanobacteria and is biobrick-compatible. It codes for RFP under synechocystis psbAB promoter.
C2.2
Construct designed from pPMQAK1 expression plasmid, which replicates both in E. coli and cyanobacteria and is biobrick-compatible. It codes for RFP under synechocystis psaA2 promoter.
C3
Construct designed from pPMQAK1 expression plasmid, which replicates both in E. coli and cyanobacteria and is biobrick-compatible. It codes for RFP under synechocystis psbAB promoter and cuts out the whole Ccdb toxin gene.
Hyperion construct
