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Team:Macquarie Australia/Protocols/Making competent Cells
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==Competent cell protocol== | ==Competent cell protocol== | ||
| - | [[File:nitrogen.jpg|500px|thumb|left| | + | [[File:nitrogen.jpg|500px|thumb|left|Competent cells were reduced to -80C via liquid nitrogen]] |
====Methods:==== | ====Methods:==== | ||
Growth of E. coli competent cells (from Inoue et al., 1990 'High efficiency transformation of Escherichia coli with plasmids', Gene, vol. 96, pp. 23-28) | Growth of E. coli competent cells (from Inoue et al., 1990 'High efficiency transformation of Escherichia coli with plasmids', Gene, vol. 96, pp. 23-28) | ||
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Revision as of 09:24, 15 August 2012
Competent cell protocol
Methods:
Growth of E. coli competent cells (from Inoue et al., 1990 'High efficiency transformation of Escherichia coli with plasmids', Gene, vol. 96, pp. 23-28)
