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Team:Cambridge/Diary/Week 8
From 2012.igem.org
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Got the results from the transformations today. Guess what - they didn't work. Given the cells are definitely competent (the transformation of Jim's YFP DNA into the e.coli seems to have worked), the master mix may be suspect. However, the positive control plates we made yesterday of sfGFP were plated on the wrong type of antibiotic, so we'll change that before we assume anything too rash. | Got the results from the transformations today. Guess what - they didn't work. Given the cells are definitely competent (the transformation of Jim's YFP DNA into the e.coli seems to have worked), the master mix may be suspect. However, the positive control plates we made yesterday of sfGFP were plated on the wrong type of antibiotic, so we'll change that before we assume anything too rash. | ||
| - | In other news, Oli and Paul began thinking about how to tune our system using an outside parameter, for use with our standardized output system. See what we did '''here ''need to link'''''. | + | In other news, Oli and Paul began thinking about how to tune our system using an outside parameter, for use with our standardized output system. It may be valuable to spend some time modelling such a system in visual GEC. See what we did '''here ''need to link'''''. |
===Tuesday=== | ===Tuesday=== | ||
Revision as of 09:27, 14 August 2012
| Week: | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 |
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Contents |
Monday
Got the results from the transformations today. Guess what - they didn't work. Given the cells are definitely competent (the transformation of Jim's YFP DNA into the e.coli seems to have worked), the master mix may be suspect. However, the positive control plates we made yesterday of sfGFP were plated on the wrong type of antibiotic, so we'll change that before we assume anything too rash.
In other news, Oli and Paul began thinking about how to tune our system using an outside parameter, for use with our standardized output system. It may be valuable to spend some time modelling such a system in visual GEC. See what we did here need to link.
Tuesday
Wednesday
Thursday
Friday
