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	===[[Team:Macquarie/Protocols/Making LB agar plates|Making liquid media, plates & buffers]]===		===[[Team:Macquarie/Protocols/Making LB agar plates|Making liquid media, plates & buffers]]===

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Revision as of 03:41, 14 August 2012

Contents 1 Tuesday 7/8/12 1.1 Making liquid media, plates & buffers 1.2 Designing Gibson Assembly Fragments 2 Tuesday 14/8/12 2.1 Making Competent Cells 2.2 Outreach Planning

Tuesday 7/8/12

(Click on headings to visit methods)

Making liquid media, plates & buffers

• Liquid LB Media

• SOC Solution

• SOB Solution

• LB Agar Plates

Ampicillin LB Agar Plates: 31 plates

Chloramphenicol LB Agar Plates: 33 Plates

Kanamyacin LB Agar Plates: 32 Plates

• TB buffer.

• TAE buffer.

• EDTA buffer.

Designing Gibson Assembly Fragments

• Haemoxygenase Gene
• Deinococcus radiodurans Bacteriophytochrome Gene
• Agrobacterium tumefaciens Bacteriophytochrome Gene

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Tuesday 14/8/12

Making Competent Cells

• Choosing Biobricks
• Making competent cells
• Transformations

Outreach Planning

• Open Day

• Secondary Education Seminars