Team:UC Chile/Cyano/Labook/week9

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<p><font face="Calibri"><font size="5">April 30 - May 6</font></font></p>
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<p><font face="Calibri"><font size="4">Monday</font></font></p>
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<br>
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<p><font face="Calibri">
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*Electrophoresis for constructs C1.1 and C1.2 assembled through Gibson. Wrong size.
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*PCR run of parts C1.1 and C1.2 for new Gibson.
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<p><font face="Calibri"><font size="4">Tuesday</font></font></p>
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<p><font face="Calibri">
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*KanR + double terminator has strange results. An analytic digestion will be done to check insertion.
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*As Gibson assembly is not working, we still need to try if pPMQAK1 works in synechocystis. We'll take out the toxic by standard assembly and transform Synechocystis afterwards.
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*Negative control of competent cells with pPMQAK1 was positive. Possible contamination.
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*Colonies in Gibson negative controls.
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Revision as of 21:25, 13 August 2012

Cyanolux & Bactomithril - Pontificia Universidad Católica de Chile, iGEM 2012


April 30 - May 6


Monday


  • Electrophoresis for constructs C1.1 and C1.2 assembled through Gibson. Wrong size.
  • PCR run of parts C1.1 and C1.2 for new Gibson.


Tuesday


  • KanR + double terminator has strange results. An analytic digestion will be done to check insertion.
  • As Gibson assembly is not working, we still need to try if pPMQAK1 works in synechocystis. We'll take out the toxic by standard assembly and transform Synechocystis afterwards.
  • Negative control of competent cells with pPMQAK1 was positive. Possible contamination.
  • Colonies in Gibson negative controls.

Retrieved from "http://2012.igem.org/Team:UC_Chile/Cyano/Labook/week9"