Team:UNITN-Trento

From 2012.igem.org

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<h1><a href="https://2012.igem.org/Team:UNITN-Trento">iGEM Trento 2012</a></h1>
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<h1><a href="https://2012.igem.org/Team:UNITN-Trento">iGEM Trento 2012</a></h1>
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<li><a href="https://2012.igem.org/Team:UNITN-Trento">Home</a></li>
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<li><a href="https://2012.igem.org/Team:UNITN-Trento">Home</a></li>
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<li><a href="https://2012.igem.org/Team:UNITN-Trento/Team">Team</a></li>
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<li><a href="https://2012.igem.org/Team:UNITN-Trento/Team">Team</a></li>
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<li><a href="#">Lab Notes</a>
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<li><a href="#">Lab Notes</a>
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<ul class="labnotes">
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<ul class="labnotes">
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<li><a href="https://2012.igem.org/Team:UNITN-Trento/Notebook">Notebook</a></li>
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<li><a href="https://2012.igem.org/Team:UNITN-Trento/Notebook">Notebook</a></li>
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<li><a href="https://2012.igem.org/Team:UNITN-Trento/Protocols">Protocols</a></li>
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<li><a href="https://2012.igem.org/Team:UNITN-Trento/Protocols">Protocols</a></li>
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<li><a href="https://2012.igem.org/Team:UNITN-Trento/Meetings">Meetings</a></li>
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<li><a href="https://2012.igem.org/Team:UNITN-Trento/Meetings">Meetings</a></li>
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<li><a href="https://2012.igem.org/Team:UNITN-Trento/Attributions">Attributions</a></li>
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<li><a href="https://2012.igem.org/Team:UNITN-Trento/Attributions">Attributions</a></li>
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<li>|</li>
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<li><a href="https://2012.igem.org/Team:UNITN-Trento/Project">Project</a></li>
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<li><a href="https://2012.igem.org/Team:UNITN-Trento/Project">Project</a></li>
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<li><a href="https://2012.igem.org/Team:UNITN-Trento/Parts">Our Parts</a></li>
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<li><a href="https://2012.igem.org/Team:UNITN-Trento/Parts">Our Parts</a></li>
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<li><a href="#">Considerations</a>
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<li><a href="#">Considerations</a>
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<li><a href="https://2012.igem.org/Team:UNITN-Trento/Safety">Safety</a></li>
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<li><a href="https://2012.igem.org/Team:UNITN-Trento/Safety">Safety</a></li>
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<li><a href="https://2012.igem.org/Team:UNITN-Trento/Outreach">Outreach</a></li>
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<li><a href="https://2012.igem.org/Team:UNITN-Trento/Outreach">Outreach</a></li>
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<li><a href="https://2012.igem.org/Team:UNITN-Trento/Blog">Blog</a></li>
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<li><a href="https://2012.igem.org/Team:UNITN-Trento/Blog">Blog</a></li>
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<li><a href="https://2012.igem.org/Team:UNITN-Trento/Gallery">Gallery</a></li>
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<li><a href="https://2012.igem.org/Team:UNITN-Trento/Gallery">Gallery</a></li>
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<li><a href="https://2012.igem.org/Team:UNITN-Trento/Partners">Partners</a></li>
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<li><a href="https://2012.igem.org/Team:UNITN-Trento/Partners">Partners</a></li>
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<li><a class="igem" href="https://2012.igem.org"><img src="http://www.jasonfweb.com/igem/img/theme/UniTN-iGEM.png" alt="iGEM" width="60px" /></a></li>
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<li><a class="igem" href="https://2012.igem.org"><img src="http://www.jasonfweb.com/igem/img/theme/UniTN-iGEM.png" alt="iGEM" width="60px" /></a></li>
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<h2 style="width: 500px;"><span>We've been diligently journaling our progress.</span> <span>Have we?</span></h2>
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<h2 style="width: 280px;"><span>We will save the World.</span> <span>One statue at a time.</span></h2>
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        <a href="#"><img src="http://www.jasonfweb.com/igem/img/theme/team-1.jpg" alt="" /></a>
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        <img src="http://www.jasonfweb.com/igem/img/theme/team-2.jpg" alt="" />
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<h2 class="tk-chippewa-falls">Hi, we're the UniTN 2012 iGEM team!</h2>
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<p>Our project will be about statues and momuments. Here in Italy there are lots of them, magnificent and important. The problem is, with the modern society going on and everything, they get all ugly. Black ugly. We want to solve that. Ah, and protect them for the future, too.</p>
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<p>You know what the cool thing is? We'll engineer bacteria just to do that!</p>
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<h4><a href="http://www.facebook.com/UniTNiGEM">Find us on Facebook<span>➞</span></a></h4>
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<div class="fb-like" data-href="https://www.facebook.com/UniTNiGEM" data-send="false" data-width="250" data-show-faces="true" data-font="lucida grande"></div>
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<li style="font-weight: bold;">Subpages:</li>
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<li><a href="#">Notebook</a></li>
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<li><a href="#">Protocols</a></li>
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<h2>Which Diary do you wanna read?</h2>
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<li><a class="one" href="javascript:void(0);"><img src="http://www.jasonfweb.com/igem/img/team/Jason.jpg" alt="" /></a><a class="one" href="javascript:void(0);"><img src="http://www.jasonfweb.com/igem/img/team/Ross.jpg" alt="" /></a><a class="one" href="javascript:void(0);"><h3>Black Crust CysE</h3></a></li>
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<li><a class="two" href="javascript:void(0);"><img src="http://www.jasonfweb.com/igem/img/team/Andrea.jpg" alt="" /></a><a href="javascript:void(0);" class="two"><img src="http://www.jasonfweb.com/igem/img/team/Gugu.jpg" alt="" /></a><a class="two" href="javascript:void(0);"><h3>Black Crust CysDes</h3></a></li>
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<li><a class="three" href="javascript:void(0);"><img src="http://www.jasonfweb.com/igem/img/team/Giaco.jpg" alt="" /></a><a href="javascript:void(0);" class="three"><img src="http://www.jasonfweb.com/igem/img/team/Anna.jpg" alt="" /></a><a class="three" href="javascript:void(0);"><h3 style="left: -220px;">Terminators</h3></a></li>
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<h2>Project Stautes</h2>
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<p>Gag ipsum dolar sit amet venenatis i dont get it good guy always feel like a sir le derp portfolio. Coffee alcohol problem? Happy asian you don't say? Super Rage dead people lois one does not simply drink blizzard captain meme computer. Homework facebook 9000 bart nother to do here woman steve jobs on avengers for 140%. Students bra dummies female true story cool crying not bad. Anonymous pokeman close enough on that bacon troll i see what you did there. Mom final week read megusta strangers really? Cereal guy le girlfriend geek male michelle just elephant win gag. Phone unsave high school a challenge accepted oh stop it, you unlock joke wodka rainbow all the things. Weekend oboma laptop panda college creepy me gusta nyan cat humor. Sister easter left admire movie vegan facepalm bear i know that feel the avengers. Gasp scared y u no top monday pizza cat impossibru note sit russia puking rainbows. Morbi.</p>
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<h3>23.11.1976</h3>
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<h4>This is a day like any other!</h4>
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<p>Le derp cat always scumbag students cool derp humor poker face. I'm Watching U win weekend crying cereal guy ba dumm tss twitter lose oboma. Computer no bad silent hill basic math le friend mother food lois people just. Nother To Do Here russia le me meme 140% hammer impossibru le girlfriend top. Donut super rage dad charlie sheen monday homework drink unlock admire yao puking rainbows. Essay hac forever alone right megusta freddie mercury aww yeah not okay vegan diablo 3. Sister movies iron man avengers ipsum facepalm men pizza scared. Cereal that house note dummies cookie monster party genius captain male. Rebecca Black asian really? No lol happy left.</p>
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<p>LOL in close enough sister strangers rebecca black simpson bart search. On superhero alcohol t-rex loki morbi bra true story lois. Genius ipsum male feel like a sir basic math monday no cuteness overload. Varius diablo 3 phone dead 140% just grey in. If You Know What I Mean men people steve jobs wodka easter always le derp yao charlie sheen star wars. Gasp laptop cereal luke a avenger ba dumm tss collection. Students peter griffin gag trolololo happy venenatis scumbag geek super rage grin. Cereal guy meme le friend lose i know that feel back puking rainbows michelle pokeman keyboard. Donut architect admire really? Scared mom joke thor derpina i'm watching u read tank twitter. Female vegan good guy troll that all the things eat homer. Dog russia top.</p>
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<p>Gag ipsum dolar sit amet venenatis i dont get it good guy always feel like a sir le derp portfolio. Coffee alcohol problem? Happy asian you don't say? Super Rage dead people lois one does not simply drink blizzard captain meme computer. Homework facebook 9000 bart nother to do here woman steve jobs on avengers for 140%. Students bra dummies female true story cool crying not bad. Anonymous pokeman close enough on that bacon troll i see what you did there. Mom final week read megusta strangers really? Cereal guy le girlfriend geek male michelle just elephant win gag. Phone unsave high school a challenge accepted oh stop it, you unlock joke wodka rainbow all the things. Weekend oboma laptop panda college creepy me gusta nyan cat humor. Sister easter left admire movie vegan facepalm bear i know that feel the avengers. Gasp scared y u no top monday pizza cat impossibru note sit russia puking rainbows. Morbi.</p>
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<p>Le derp cat always scumbag students cool derp humor poker face. I'm Watching U win weekend crying cereal guy ba dumm tss twitter lose oboma. Computer no bad silent hill basic math le friend mother food lois people just. Nother To Do Here russia le me meme 140% hammer impossibru le girlfriend top. Donut super rage dad charlie sheen monday homework drink unlock admire yao puking rainbows. Essay hac forever alone right megusta freddie mercury aww yeah not okay vegan diablo 3. Sister movies iron man avengers ipsum facepalm men pizza scared. Cereal that house note dummies cookie monster party genius captain male. Rebecca Black asian really? No lol happy left.</p>
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<h3>23.11.1976</h3>
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<p>LOL in close enough sister strangers rebecca black simpson bart search. On superhero alcohol t-rex loki morbi bra true story lois. Genius ipsum male feel like a sir basic math monday no cuteness overload. Varius diablo 3 phone dead 140% just grey in. If You Know What I Mean men people steve jobs wodka easter always le derp yao charlie sheen star wars. Gasp laptop cereal luke a avenger ba dumm tss collection. Students peter griffin gag trolololo happy venenatis scumbag geek super rage grin. Cereal guy meme le friend lose i know that feel back puking rainbows michelle pokeman keyboard. Donut architect admire really? Scared mom joke thor derpina i'm watching u read tank twitter. Female vegan good guy troll that all the things eat homer. Dog russia top.</p>
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<p>Gag ipsum dolar sit amet venenatis i dont get it good guy always feel like a sir le derp portfolio. Coffee alcohol problem? Happy asian you don't say? Super Rage dead people lois one does not simply drink blizzard captain meme computer. Homework facebook 9000 bart nother to do here woman steve jobs on avengers for 140%. Students bra dummies female true story cool crying not bad. Anonymous pokeman close enough on that bacon troll i see what you did there. Mom final week read megusta strangers really? Cereal guy le girlfriend geek male michelle just elephant win gag. Phone unsave high school a challenge accepted oh stop it, you unlock joke wodka rainbow all the things. Weekend oboma laptop panda college creepy me gusta nyan cat humor. Sister easter left admire movie vegan facepalm bear i know that feel the avengers. Gasp scared y u no top monday pizza cat impossibru note sit russia puking rainbows. Morbi.</p>
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<h3>23.11.1976</h3>
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<h4>This is a day like any other!</h4>
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<p>Le derp cat always scumbag students cool derp humor poker face. I'm Watching U win weekend crying cereal guy ba dumm tss twitter lose oboma. Computer no bad silent hill basic math le friend mother food lois people just. Nother To Do Here russia le me meme 140% hammer impossibru le girlfriend top. Donut super rage dad charlie sheen monday homework drink unlock admire yao puking rainbows. Essay hac forever alone right megusta freddie mercury aww yeah not okay vegan diablo 3. Sister movies iron man avengers ipsum facepalm men pizza scared. Cereal that house note dummies cookie monster party genius captain male. Rebecca Black asian really? No lol happy left.</p>
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<p>LOL in close enough sister strangers rebecca black simpson bart search. On superhero alcohol t-rex loki morbi bra true story lois. Genius ipsum male feel like a sir basic math monday no cuteness overload. Varius diablo 3 phone dead 140% just grey in. If You Know What I Mean men people steve jobs wodka easter always le derp yao charlie sheen star wars. Gasp laptop cereal luke a avenger ba dumm tss collection. Students peter griffin gag trolololo happy venenatis scumbag geek super rage grin. Cereal guy meme le friend lose i know that feel back puking rainbows michelle pokeman keyboard. Donut architect admire really? Scared mom joke thor derpina i'm watching u read tank twitter. Female vegan good guy troll that all the things eat homer. Dog russia top.</p>
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<p>Gag ipsum dolar sit amet venenatis i dont get it good guy always feel like a sir le derp portfolio. Coffee alcohol problem? Happy asian you don't say? Super Rage dead people lois one does not simply drink blizzard captain meme computer. Homework facebook 9000 bart nother to do here woman steve jobs on avengers for 140%. Students bra dummies female true story cool crying not bad. Anonymous pokeman close enough on that bacon troll i see what you did there. Mom final week read megusta strangers really? Cereal guy le girlfriend geek male michelle just elephant win gag. Phone unsave high school a challenge accepted oh stop it, you unlock joke wodka rainbow all the things. Weekend oboma laptop panda college creepy me gusta nyan cat humor. Sister easter left admire movie vegan facepalm bear i know that feel the avengers. Gasp scared y u no top monday pizza cat impossibru note sit russia puking rainbows. Morbi.</p>
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<p>Le derp cat always scumbag students cool derp humor poker face. I'm Watching U win weekend crying cereal guy ba dumm tss twitter lose oboma. Computer no bad silent hill basic math le friend mother food lois people just. Nother To Do Here russia le me meme 140% hammer impossibru le girlfriend top. Donut super rage dad charlie sheen monday homework drink unlock admire yao puking rainbows. Essay hac forever alone right megusta freddie mercury aww yeah not okay vegan diablo 3. Sister movies iron man avengers ipsum facepalm men pizza scared. Cereal that house note dummies cookie monster party genius captain male. Rebecca Black asian really? No lol happy left.</p>
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<p>LOL in close enough sister strangers rebecca black simpson bart search. On superhero alcohol t-rex loki morbi bra true story lois. Genius ipsum male feel like a sir basic math monday no cuteness overload. Varius diablo 3 phone dead 140% just grey in. If You Know What I Mean men people steve jobs wodka easter always le derp yao charlie sheen star wars. Gasp laptop cereal luke a avenger ba dumm tss collection. Students peter griffin gag trolololo happy venenatis scumbag geek super rage grin. Cereal guy meme le friend lose i know that feel back puking rainbows michelle pokeman keyboard. Donut architect admire really? Scared mom joke thor derpina i'm watching u read tank twitter. Female vegan good guy troll that all the things eat homer. Dog russia top.</p>
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<p>Gag ipsum dolar sit amet venenatis i dont get it good guy always feel like a sir le derp portfolio. Coffee alcohol problem? Happy asian you don't say? Super Rage dead people lois one does not simply drink blizzard captain meme computer. Homework facebook 9000 bart nother to do here woman steve jobs on avengers for 140%. Students bra dummies female true story cool crying not bad. Anonymous pokeman close enough on that bacon troll i see what you did there. Mom final week read megusta strangers really? Cereal guy le girlfriend geek male michelle just elephant win gag. Phone unsave high school a challenge accepted oh stop it, you unlock joke wodka rainbow all the things. Weekend oboma laptop panda college creepy me gusta nyan cat humor. Sister easter left admire movie vegan facepalm bear i know that feel the avengers. Gasp scared y u no top monday pizza cat impossibru note sit russia puking rainbows. Morbi.</p>
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<h3>23.11.1976</h3>
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<h4>This is a day like any other!</h4>
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<p>Le derp cat always scumbag students cool derp humor poker face. I'm Watching U win weekend crying cereal guy ba dumm tss twitter lose oboma. Computer no bad silent hill basic math le friend mother food lois people just. Nother To Do Here russia le me meme 140% hammer impossibru le girlfriend top. Donut super rage dad charlie sheen monday homework drink unlock admire yao puking rainbows. Essay hac forever alone right megusta freddie mercury aww yeah not okay vegan diablo 3. Sister movies iron man avengers ipsum facepalm men pizza scared. Cereal that house note dummies cookie monster party genius captain male. Rebecca Black asian really? No lol happy left.</p>
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<p>LOL in close enough sister strangers rebecca black simpson bart search. On superhero alcohol t-rex loki morbi bra true story lois. Genius ipsum male feel like a sir basic math monday no cuteness overload. Varius diablo 3 phone dead 140% just grey in. If You Know What I Mean men people steve jobs wodka easter always le derp yao charlie sheen star wars. Gasp laptop cereal luke a avenger ba dumm tss collection. Students peter griffin gag trolololo happy venenatis scumbag geek super rage grin. Cereal guy meme le friend lose i know that feel back puking rainbows michelle pokeman keyboard. Donut architect admire really? Scared mom joke thor derpina i'm watching u read tank twitter. Female vegan good guy troll that all the things eat homer. Dog russia top.</p>
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<p>Gag ipsum dolar sit amet venenatis i dont get it good guy always feel like a sir le derp portfolio. Coffee alcohol problem? Happy asian you don't say? Super Rage dead people lois one does not simply drink blizzard captain meme computer. Homework facebook 9000 bart nother to do here woman steve jobs on avengers for 140%. Students bra dummies female true story cool crying not bad. Anonymous pokeman close enough on that bacon troll i see what you did there. Mom final week read megusta strangers really? Cereal guy le girlfriend geek male michelle just elephant win gag. Phone unsave high school a challenge accepted oh stop it, you unlock joke wodka rainbow all the things. Weekend oboma laptop panda college creepy me gusta nyan cat humor. Sister easter left admire movie vegan facepalm bear i know that feel the avengers. Gasp scared y u no top monday pizza cat impossibru note sit russia puking rainbows. Morbi.</p>
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<p>Le derp cat always scumbag students cool derp humor poker face. I'm Watching U win weekend crying cereal guy ba dumm tss twitter lose oboma. Computer no bad silent hill basic math le friend mother food lois people just. Nother To Do Here russia le me meme 140% hammer impossibru le girlfriend top. Donut super rage dad charlie sheen monday homework drink unlock admire yao puking rainbows. Essay hac forever alone right megusta freddie mercury aww yeah not okay vegan diablo 3. Sister movies iron man avengers ipsum facepalm men pizza scared. Cereal that house note dummies cookie monster party genius captain male. Rebecca Black asian really? No lol happy left.</p>
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<article>
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<h3>23.11.1976</h3>
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<h4>This is a day like any other!</h4>
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<p>LOL in close enough sister strangers rebecca black simpson bart search. On superhero alcohol t-rex loki morbi bra true story lois. Genius ipsum male feel like a sir basic math monday no cuteness overload. Varius diablo 3 phone dead 140% just grey in. If You Know What I Mean men people steve jobs wodka easter always le derp yao charlie sheen star wars. Gasp laptop cereal luke a avenger ba dumm tss collection. Students peter griffin gag trolololo happy venenatis scumbag geek super rage grin. Cereal guy meme le friend lose i know that feel back puking rainbows michelle pokeman keyboard. Donut architect admire really? Scared mom joke thor derpina i'm watching u read tank twitter. Female vegan good guy troll that all the things eat homer. Dog russia top.</p>
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<div class="footer">
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<div class="mw">
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<h2 class="tk-chippewa-falls">Project Codename: Statuary</h2><div class="medium_ribbon" ></div>
-
<ul>
+
<p>Statues and monuments all over the world are not enjoyed to the extent that they should be because they are covered in a disfiguring black crust. <br /> A practical solution would be if a cheap mechanism were available to continually remove the pollutant molecules that adhere to the surface of statues. To build such a system, we are engineering E. coli to dissolve the disfiguring black crust, which consists mainly of CaSO4 (i.e. gypsum). <br /> More specifically, we are constructing an aerobic sulfur reducing pathway composed of a mutant E. coli serine acetyltransferase (CysE) that is insensitive to feedback inhibition and a Treponema denticola cysteine desulfhydrase. CysE acetylates serine to give a precursor for cysteine synthesis, which also activates a sulfate assimilation pathway. This pathway should then mobilize the SO4 of the black crust to make cysteine. Finally, the cysteine desulfhydrase converts cysteine to H2S, pyruvate, and ammonia, thus completing the transformation of black crust sulfate to hydrogen sulfide gas.</p>
-
<li><a href="https://2012.igem.org">iGEM Main Page</a></li>
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<li><a href="https://2012.igem.org/wiki/index.php?title=Talk:Team:UNITN-Trento&action=edit&redlink=1">Discussion</a></li>
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<li><a href="https://2012.igem.org/wiki/index.php?title=Team:UNITN-Trento&action=edit">Edit</a></li>
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+
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<li><a href="#">Teams</a></li>
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<li>|</li>
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-
<li><a href="https://2012.igem.org/Special:RecentChanges">Recent Changes</a></li>
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<!--<li><a href="https://2012.igem.org/Special:WhatLinksHere/Team:UNITN-Trento">What links here</a></li>-->
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<li><a href="https://2012.igem.org/Special:RecentChangesLinked/Team:UNITN-Trento">Related Changes</a></li>
+
-
<li><a href="https://2012.igem.org/Special:SpecialPages">Special Pages</a></li>
+
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<li><a href="https://2012.igem.org/Special:Preferences">My Preferences</a></li>
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<!--<li><a href="https://2012.igem.org/wiki/index.php?title=Team:UNITN-Trento&printable=yes">Printable Version</a></li>
+
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<li><a href="https://2012.igem.org/2012.igem.org:Privacy_policy">Privacy policy</a></li>
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<li><a href="https://2012.igem.org/2012.igem.org:General_disclaimer">Disclaimer</a></li>
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-
<p><span class="toggle">Toggle controls</span> |  Design by Jason Fontana. Powered by <a href="http://www.mediawiki.org/wiki/MediaWiki">MediaWiki</a>. This website is under the <a href="http://creativecommons.org/licenses/by/3.0/">CC Attribution licence</a>.<br /><span style="left: 370px;
+
<div class="grid_11 projects">
-
position: relative;">Follow our daily journal on <a class="facebook" href="http://www.facebook.com/UniTNiGEM" target="_blank"> Facebook </a>!</span></p>
+
<h2 class="tk-chippewa-falls">Project Codename: Terminator 5</h2><div class="medium_ribbon" ></div>
 +
<p>Surprisingly little standardization of T7 bacteriophage derived parts has occurred, despite the prevalence of T7 parts in recombinant technologies. <br /> Additionally, the compatibility between T7 and E. coli derived parts has not been explored.  For example, how efficient are T7 transcriptional terminators with E. coli RNA polymerase and is an E. coli transcriptional terminator effective with T7 RNA polymerase?  <br />To better understand the compatibility of the two systems and to begin to better characterize T7 biological parts, we are measuring the termination efficiencies of T7 and E. coli transcriptional terminators with E. coli and T7 RNA polymerases. <br /> To facilitate our measurements, we have developed a new biobrick compatible vector for ratiometric analyses by fluorescence spectroscopy and cell sorting.</p>
 +
</div>
 +
 +
</div> <!--end two-->
 +
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<div class="three">
 +
<div class="grid_8 sec" >
 +
 +
<h3><a href="https://2012.igem.org/Team:UNITN-Trento/Project">Project <span>➞</span></a></h3><div class="small_ribbon" ></div>
 +
<p>Our awesome project. We have a brief overview of what we're doing. This the place to look if you want more details.</p>
 +
</div>
</div>
-
<div class="sponsor">
+
<div class="grid_8 sec" >
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<a href="http://www.fondazionecassaruraleditrento.it"><img src="http://www.jasonfweb.com/igem/img/sponsor/CRT.jpg" alt="Cassa Rurale di Trento" /></a>
+
-
<a href="http://www.unitn.it/en/cibio"><img src="http://www.jasonfweb.com/igem/img/sponsor/CIBIO.png" alt="CIBIO"/></a>
+
<h3><a href="https://2012.igem.org/Team:UNITN-Trento/Blog">Blog <span>➞</span></a></h3><div class="small_ribbon" ></div>
-
<!--<a href="http://www.unitn.it/en"><img src="http://www.jasonfweb.com/igem/img/sponsor/UniTN.JPG" alt="Università degli Studi di Trento"/></a>-->
+
<p>Our daily mumbling. The Journal is not ready yet, but we have a blog with our impressions. Also, check out our <a class="facebook" href="http://www.facebook.com/UniTNiGEM" target="_blank">Facebook page</a>! </p>
</div>
</div>
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<div class="unitn">
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<div class="grid_8 sec" >
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<img src="http://www.jasonfweb.com/igem/img/sponsor/UniTN.JPG" alt="Università degli Studi di Trento"/>
+
 +
<h3><a href="https://2012.igem.org/Team:UNITN-Trento/Team">Team <span>➞</span></a></h3><div class="small_ribbon" ></div>
 +
<p>Our team members. <br /> We're pretty cool, so stop by.</p>
</div>
</div>
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<marquee class="rolling" scrollamount="3">We have successfully cloned and sequenced all our planned parts! Thank you to Genechron that supported us by sequencing 204 samples for free! &nbsp;&nbsp;&nbsp;&nbsp;-&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;iGEM Trento will be participating in the "Researchers' Night 2012", a European Community sponsored outreach activity. The event will be held in Trento on September 28.</marquee>
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<p><span class="toggle">Toggle controls</span>  |  Design by Jason Fontana. Powered by <a href="http://www.mediawiki.org/wiki/MediaWiki">MediaWiki</a>. This website is under the <a href="http://creativecommons.org/licenses/by/3.0/">CC Attribution licence</a>.<br /><span style="left: 370px;
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position: relative;">Follow our daily journal on <a class="facebook" href="http://www.facebook.com/UniTNiGEM" target="_blank"> Facebook </a>!</span></p>
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</div>
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<div class="sponsor">
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<a href="http://www.fondazionecassaruraleditrento.it"><img src="http://www.jasonfweb.com/igem/img/sponsor/CRT.jpg" alt="Cassa Rurale di Trento" /></a>
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Revision as of 14:22, 12 August 2012

iGEM Trento 2012 @jasonfweb

iGEM Trento 2012

We will save the World. One statue at a time.

Hi, we're the UniTN 2012 iGEM team!

Our project will be about statues and momuments. Here in Italy there are lots of them, magnificent and important. The problem is, with the modern society going on and everything, they get all ugly. Black ugly. We want to solve that. Ah, and protect them for the future, too.

You know what the cool thing is? We'll engineer bacteria just to do that!

Project Codename: Statuary

Statues and monuments all over the world are not enjoyed to the extent that they should be because they are covered in a disfiguring black crust.
A practical solution would be if a cheap mechanism were available to continually remove the pollutant molecules that adhere to the surface of statues. To build such a system, we are engineering E. coli to dissolve the disfiguring black crust, which consists mainly of CaSO4 (i.e. gypsum).
More specifically, we are constructing an aerobic sulfur reducing pathway composed of a mutant E. coli serine acetyltransferase (CysE) that is insensitive to feedback inhibition and a Treponema denticola cysteine desulfhydrase. CysE acetylates serine to give a precursor for cysteine synthesis, which also activates a sulfate assimilation pathway. This pathway should then mobilize the SO4 of the black crust to make cysteine. Finally, the cysteine desulfhydrase converts cysteine to H2S, pyruvate, and ammonia, thus completing the transformation of black crust sulfate to hydrogen sulfide gas.

Project Codename: Terminator 5

Surprisingly little standardization of T7 bacteriophage derived parts has occurred, despite the prevalence of T7 parts in recombinant technologies.
Additionally, the compatibility between T7 and E. coli derived parts has not been explored. For example, how efficient are T7 transcriptional terminators with E. coli RNA polymerase and is an E. coli transcriptional terminator effective with T7 RNA polymerase?
To better understand the compatibility of the two systems and to begin to better characterize T7 biological parts, we are measuring the termination efficiencies of T7 and E. coli transcriptional terminators with E. coli and T7 RNA polymerases.
To facilitate our measurements, we have developed a new biobrick compatible vector for ratiometric analyses by fluorescence spectroscopy and cell sorting.

Project ➞

Our awesome project. We have a brief overview of what we're doing. This the place to look if you want more details.

Blog ➞

Our daily mumbling. The Journal is not ready yet, but we have a blog with our impressions. Also, check out our !

Team ➞

Our team members.
We're pretty cool, so stop by.

Breaking News:

We have successfully cloned and sequenced all our planned parts! Thank you to Genechron that supported us by sequencing 204 samples for free!     -     iGEM Trento will be participating in the "Researchers' Night 2012", a European Community sponsored outreach activity. The event will be held in Trento on September 28.

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Università degli Studi di Trento

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