Team:Amsterdam/project/diary/lab
From 2012.igem.org
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- | <u><h4>26/04/2012</h4></u> | + | <u><center><h4>26/04/2012</h4></center></u> |
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<p> | <p> | ||
We arrive at the lab to get started! First items on the agenda are getting settled at the lab and acquiring our needed equipment. As a bonus we do a transformation need tot test the Gibson method we will be using throughout these months. | We arrive at the lab to get started! First items on the agenda are getting settled at the lab and acquiring our needed equipment. As a bonus we do a transformation need tot test the Gibson method we will be using throughout these months. | ||
- | We will do this by | + | We will do this by cloning a GFP into a pET28 vector using our transformation protocol. |
</p> | </p> | ||
<p> | <p> | ||
- | 27/04/2012 | + | <u><center><h4>27/04/2012</h4></center></u> |
</p> | </p> | ||
<p> | <p> | ||
+ | Succesfully transformed the plasmids. Ordered the primers for our gibson assembly and started to prepare things. Aliquoting our phusion and gisbon master mix. | ||
</p> | </p> | ||
<p> | <p> | ||
+ | <u><center><h4>07/05/2012</h4></center></u> | ||
</p> | </p> | ||
+ | |||
+ | <p> | ||
+ | Preparing O/N cultures which can be taken out of 37C in the weekend by a friend | ||
+ | </p> | ||
+ | |||
+ | <p> | ||
+ | <u><center><h4>08/05/2012</h4></center></u> | ||
+ | </p> | ||
+ | |||
+ | <p> | ||
+ | Our friend former igem member Yuri of 2011 will take out our O/N cultures. | ||
+ | </p> | ||
+ | |||
+ | <p> | ||
+ | <u><center><h4>09/05/2012</h4></center></u> | ||
+ | </p> | ||
+ | |||
+ | <p> | ||
+ | Yuri forgot... Start again. Note; rely on ourselves | ||
+ | </p> | ||
+ | |||
+ | <p> | ||
+ | <u><center><h4>10/05/2012</h4></center></u> | ||
+ | </p> | ||
+ | |||
+ | <p> | ||
+ | The primers arrived. we reconstituded them and tried them on the plasmids after mini-prepping our O/N culture. | ||
+ | Gel: | ||
+ | Starting our first PCR. | ||
+ | </p> | ||
+ | |||
+ | |||
+ | |||
+ | |||
</div> | </div> | ||
{{Team:Amsterdam/Foot}} | {{Team:Amsterdam/Foot}} |
Revision as of 13:48, 3 August 2012
Contents |
Lab Journal
26/04/2012
We arrive at the lab to get started! First items on the agenda are getting settled at the lab and acquiring our needed equipment. As a bonus we do a transformation need tot test the Gibson method we will be using throughout these months. We will do this by cloning a GFP into a pET28 vector using our transformation protocol.
27/04/2012
Succesfully transformed the plasmids. Ordered the primers for our gibson assembly and started to prepare things. Aliquoting our phusion and gisbon master mix.
07/05/2012
Preparing O/N cultures which can be taken out of 37C in the weekend by a friend
08/05/2012
Our friend former igem member Yuri of 2011 will take out our O/N cultures.
09/05/2012
Yuri forgot... Start again. Note; rely on ourselves
10/05/2012
The primers arrived. we reconstituded them and tried them on the plasmids after mini-prepping our O/N culture. Gel: Starting our first PCR.