Team:Leicester/August2012

From 2012.igem.org

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<p>(9:30 am) Several members of the team are given wrong start times, so only 1 student arrived in the lab at 9:30, however with the work needed to be done all the rest of the team are quickly on their way.</p>
<p>(9:30 am) Several members of the team are given wrong start times, so only 1 student arrived in the lab at 9:30, however with the work needed to be done all the rest of the team are quickly on their way.</p>
<p>(10:00 am) Today the team is going to start creating a DNA library of the ''Pseudomonas. sp'' so that once the NB26 strain arrives it can be compared straight away to give an idea which genes could be involved in the degredation of polystyrene and to help start narrowing our search.</p>
<p>(10:00 am) Today the team is going to start creating a DNA library of the ''Pseudomonas. sp'' so that once the NB26 strain arrives it can be compared straight away to give an idea which genes could be involved in the degredation of polystyrene and to help start narrowing our search.</p>
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<p> The team starts by creating the control plates with ''E. coli''. The 3 plates created are: plain mineral media, and two of the minimal media with 5% polystyrene sprinkled on top. The ''E. coli'' is being plated on the plain media as well as one of the polystyrene sprinkled plates.</p>
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<p> To create the library a few things need to be known: Is the bacteria gram positive or negative? Is the DNA chromosomal or plasmid? How much DNA is going to be extracted? All ''Pseudomonas'' is gram negative, and the team wants to extract as much of the ..... DNA as possible. With all these answered the group can collect the right DNA extraction kit and start working.</p>
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Revision as of 09:04, 1 August 2012

    Wednesday 1st August 2012

(9:30 am) Several members of the team are given wrong start times, so only 1 student arrived in the lab at 9:30, however with the work needed to be done all the rest of the team are quickly on their way.

(10:00 am) Today the team is going to start creating a DNA library of the ''Pseudomonas. sp'' so that once the NB26 strain arrives it can be compared straight away to give an idea which genes could be involved in the degredation of polystyrene and to help start narrowing our search.

To create the library a few things need to be known: Is the bacteria gram positive or negative? Is the DNA chromosomal or plasmid? How much DNA is going to be extracted? All ''Pseudomonas'' is gram negative, and the team wants to extract as much of the ..... DNA as possible. With all these answered the group can collect the right DNA extraction kit and start working.

    Thursday 2nd August 2012

No entry for this date.

    Friday 3rd August 2012

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    Saturday 4th August 2012

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    Sunday 5th August 2012

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    Monday 6th August 2012

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    Tuesday 7th August 2012

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    Wednesday 8th August 2012

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    Thursday 9th August 2012

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    Friday 10th August 2012

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    Saturday 11th August 2012

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    Sunday 12th August 2012

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    Monday 13th August 2012

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    Tuesday 14th August 2012

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    Wedesnday 15th August 2012

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    Thursday 16th August 2012

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    Friday 17th August 2012

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    Saturday 18th August 2012

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    Sunday 19th August 2012

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    Monday 20th August 2012

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    Tuesday 21st August 2012

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    Wedesnday 22nd August 2012

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    Thursday 23rd August 2012

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    Friday 24th August 2012

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    Saturday 25th August 2012

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    Sunday 26th August 2012

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    Monday 27th August 2012

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    Tuesday 28th August 2012

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    Wednesday 29th August 2012

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    Tuesday 30th August 2012

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