Team:Trieste

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          <title>Team iGEM Trieste 2012</title>
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    <h1 id="h1_lf" class="main_tit"><div>Our Project</div></h1>
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            <h1 id="h1_mn" class="main_tit"><div>Latest news</div></h1>
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            <h1 id="h1_rt" class="main_tit"><div>Our team</div></h1>
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<h2>WE PRESENT YOU THE JOLLY JOCARE</h2>
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<h3>&nbsp;</h3>
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Recent studies have evidenced that having a beneficial and healthy
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<h1>Team iGEM Trieste 2012</h1>
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intestinal microflora is very important for human health. Our
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aim is to modify a bacteria normally found in human gut and
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create a safe, controllable and versatile molecular platform
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which can be used to produce a wide range of molecules leading
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to a beneficial probiotic. For this purpose we have chosen the
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E. coli strain Nissle 1917 which has been used for many years as
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a probiotic. We designed a robust gene guard system regulated
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        <h1>Abstract</h1>
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by a novel and easy to control inducible cumate switch that
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activates the production of a human antimicrobial peptide LL-37
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                <h2 style="background-color: white;margin: 0px; padding: 10px;text-align:center;">A safe synthetic probiotic expression system for antibodies.</h2>
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that can kill the bacteria and also avoid horizontal transfer.
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<p>Noroviruses (NoV) are cause of epidemic outbreaks of gastrointestinal illness. Even if they are considered as a moderate threat – classified as category B pathogens by the National Institute of Allergy and Infectious diseases<sup><a href="#footnote1">[1]</a></sup> – the spreading speed of these pathogens is dramatic: rates of reported NoV outbreaks reach 21 million cases of acute gastroenteritis per year, 70.000 of those need hospitalization and 800 die<sup><a href="#footnote2">[2]</a></sup>. This is due to a rapid person-to-person transmission in semi-closed places like hospital, school and public transports. Moreover it seems that it is really difficult to develop an immunological memory against NoV that avoids further illness.
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    The safe probiotic constructed here can be used to produce nutritious, preventive or therapeutic molecules. For example, we have used it to produce an antibody against the emerging virus, Norovirus.
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Our project aims to build a probiotic bacteria that helps the immune system to fight this pathogenic agent. First of all we had to choose a suitable bacterial strain for the gut environment that can easily be manipulated. Than we had to face an important issue: safety. For this purpose we designed a robust gene guard system regulated by a novel inducible switch and by an human antimicrobial peptide that can destroy, in any moment, the bacteria and avoid the transgene horizontal exchange.
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Finally we will provide to our bacteria a weapon able to capture and inactivate our target thanks to a synthetic minibody fused to a protein membrane. This feature can be slightly modified to target any other pathogenic agent with enteric tropism.
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                    <span id="footnote1">[1] <a href="http://www.niaid.nih.gov/topics/biodefenserelated/biodefense/pages/cata.aspx">http://www.niaid.nih.gov/topics/biodefenserelated/biodefense/pages/cata.aspx</a></span><br />
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                    <span id="footnote2">[2] <a href="http://www.cdc.gov/norovirus/trends-outbreaks.html">http://www.cdc.gov/norovirus/trends-outbreaks.html</a></span>
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                            <div class="title"><a href="#">Site activity</a></div>
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                            <div class="date">06/09/2012</div>
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                            <div class="intro">Safety issues and abstract posted.</div>
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    <div class="read_more"><a href="#">a</a></div>
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                            <div class="title"><a href="#">NEXT</a></div>
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                            <div class="date">04/09/2012</div>
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HumanPracticeNEXT: we created the certificate of attendance for
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the kids who will participate to our activity. We also finished
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making puzzles for the youngest!
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                            <div class="title"><a href="#">Initiating BioBrick tests</a></div>
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                            <div class="date">03/09/2012</div>
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                            <div class="intro">We are starting to test and characterize our first BioBricks!</div>
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    <div class="read_more"><a href="#">a</a></div>
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                <img src="https://static.igem.org/mediawiki/2012/b/b0/Team_trieste.jpg" alt="Team iGEM 2012" id="igem_team" />
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<h2>Contact us</h2>
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<p>For other information, write to:</p>
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                    <a href="mailto:igem2012@gmail.com" class="btn">igem2012@gmail.com</a>
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                    Follow us also:
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Revision as of 18:43, 7 September 2012

Our Project

Latest news

Our team

WE PRESENT YOU THE JOLLY JOCARE

Recent studies have evidenced that having a beneficial and healthy intestinal microflora is very important for human health. Our aim is to modify a bacteria normally found in human gut and create a safe, controllable and versatile molecular platform which can be used to produce a wide range of molecules leading to a beneficial probiotic. For this purpose we have chosen the E. coli strain Nissle 1917 which has been used for many years as a probiotic. We designed a robust gene guard system regulated by a novel and easy to control inducible cumate switch that activates the production of a human antimicrobial peptide LL-37 that can kill the bacteria and also avoid horizontal transfer.

The safe probiotic constructed here can be used to produce nutritious, preventive or therapeutic molecules. For example, we have used it to produce an antibody against the emerging virus, Norovirus.

Site activity
06/09/2012
Safety issues and abstract posted.
a
NEXT
04/09/2012
HumanPracticeNEXT: we created the certificate of attendance for the kids who will participate to our activity. We also finished making puzzles for the youngest!
a
Initiating BioBrick tests
03/09/2012
We are starting to test and characterize our first BioBricks!
a
Team iGEM 2012

Contact us

For other information, write to:

igem2012@gmail.com
Università degli studi di Trieste ICGEB Illy Fondazione Cassa di Risparmio
iGEM 2012 iGEM 2012 iGEM 2012 iGEM 2012 iGEM 2012 iGEM 2012
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