Team:USTC-China/temp00

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<html lang="en"><head><meta http-equiv="Content-Type" content="text/html; charset=UTF-8"><title>Project</title><meta name="Generator" content="Serif WebPlus X6"><meta name="viewport" content="width=1000"><script type="text/javascript" src="wpscripts/jquery.js"></script><script type="text/javascript" src="wpscripts/jquery.wpslider.js"></script><style type="text/css">body{margin:0;padding:0;}.Feature2-P{    margin:0.0px 0.0px 0.0px 0.0px; text-align:center; font-weight:400;}.Body3-P{    margin:0.0px 0.0px 0.0px 0.0px; text-align:center; font-weight:400;}.Logo-Text22-C{    font-family:"Arial", sans-serif; font-weight:700; color:#f9fff2; font-size:21.0px; line-height:1.19em;}.Body-12-C{    font-family:"Arial", sans-serif; color:#f9fff2; font-size:11.0px; line-height:1.27em;}.Normal-_Web_-C{    font-family:"Arial", sans-serif; font-size:13.0px; line-height:1.23em;}.Placeholder-C{    font-family:"Arial", sans-serif; color:#f9fff2; font-size:13.0px; line-height:1.23em;}.Sub-Heading-2-C{    font-family:"Trebuchet MS", sans-serif; 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height:1587px;"><div style="background-color:transparent;margin-left:auto;margin-right:auto;position:relative;width:1000px;height:1587px;"><img src="wpimages/wp93046f38_06.png" border="0" width="980" height="130" alt="" style="position:absolute;left:10px;top:0px;"><div style="position:absolute;left:121px;top:45px;width:235px;height:29px;overflow:hidden;"><p class="Wp-Logo-Text22-P"><span class="Logo-Text22-C">COMPANY NAME</span></p></div><img src="wpimages/wp138ec08c_06.png" border="0" width="74" height="66" alt="" style="position:absolute;left:40px;top:0px;"><img src="wpimages/wp08e3906b_06.png" border="0" width="976" height="85" alt="" style="position:absolute;left:12px;top:1502px;"><div style="position:absolute;left:53px;top:1537px;width:539px;height:35px;overflow:hidden;"><p class="Wp-Body-12-P"><span class="Body-12-C">www.ustc.edu.cn</span></p></div><div style="position:absolute;left:20px;top:79px;width:960px;height:40px;background-image:url('wpimages/wp52646929_06.png');">    <a href="index.html" id="nav_171_B1" class="Button1" style="display:block;position:absolute;left:208px;top:0px;width:134px;height:40px;"><span>Home</span></a>    <a href="page4.html" id="nav_171_B2" class="Button1" style="display:block;position:absolute;left:345px;top:0px;width:134px;height:40px;"><span>Project&nbsp;description</span></a>    <a href="page3.html" id="nav_171_B3" class="Button2" style="display:block;position:absolute;left:482px;top:0px;width:134px;height:40px;"><span>Project</span></a>    <a href="page2.html" id="nav_171_B4" class="Button1" style="display:block;position:absolute;left:619px;top:0px;width:134px;height:40px;"><span>Human&nbsp;practice</span></a></div><img src="wpimages/wp1404d01f_06.png" border="0" width="980" height="1068" title="" alt="Backgrounds&#10;" style="position:absolute;left:10px;top:451px;"><div style="position:absolute;left:305px;top:622px;width:635px;height:103px;overflow:hidden;"><p class="Wp-Normal-_Web_-P" style="margin-top:0px"><span class="Normal-_Web_-C">Since the fermentation industry occurred, the phage infection has become a big threat    to engineering bacteria. During mass production of dairy products, enzymes, amino    acids, antibiotics and pesticides, the results are unimaginable if the bacterias    are infected by bacteriophages . The bacterias will die abnormally and the infection    will spread quickly. Without any efficient action, tremendous loss will lie ahead.    Nowadays, common strategies for avoiding this catastrophe is to control the conditions    of the fermentation strictly. Also, people keep selecting strains against phages.    But all the strategies are time consuming and far from once for all. </span></p><p class="Wp-Normal-_Web_-P" style="margin-top:0px"><span class="Normal-_Web_-C">However, the engineering bacteria 2012 USTC-<wbr>China iGEMers hope to cultivate both    protecting themselves when bacteriophage invades the strain and focusing on producing    products without consuming extra energy when their living environment is safe. In    this case, the quality of fermented products can be guaranteed and the profits of    fermentation industry can be enormously improved. </span></p><p class="Wp-Body3-P"><span class="Placeholder-C"><br></span></p></div><div style="position:absolute;left:60px;top:399px;width:158px;height:22px;overflow:hidden;"><p class="Wp-Body3-P"><span class="Wp-Hyperlink2-C Sz-Hyperlink2-C-130px">Abstract</span></p></div><img src="wpimages/wp2b2acbbd_06.png" border="0" width="201" height="1" alt="" style="position:absolute;left:60px;top:392px;"><img src="wpimages/wp2b2acbbd_06.png" border="0" width="201" height="1" alt="" style="position:absolute;left:60px;top:423px;"><div style="position:absolute;left:60px;top:430px;width:158px;height:22px;overflow:hidden;"><p class="Wp-Body3-P"><span class="Wp-Hyperlink2-C Sz-Hyperlink2-C-130px"><a class="Wp-Hyperlink2-C-H" href="#" style="text-decoration:none;">Experiment plan</a></span></p></div><img src="wpimages/wp2b2acbbd_06.png" border="0" width="201" height="1" alt="" style="position:absolute;left:60px;top:454px;"><div style="position:absolute;left:60px;top:461px;width:158px;height:22px;overflow:hidden;"><p class="Wp-Body3-P"><span class="Wp-Hyperlink2-C Sz-Hyperlink2-C-130px"><a class="Wp-Hyperlink2-C-H" href="#" style="text-decoration:none;">Modeling </a></span></p></div><img src="wpimages/wp2b2acbbd_06.png" border="0" width="201" height="1" alt="" style="position:absolute;left:60px;top:485px;"><div style="position:absolute;left:60px;top:492px;width:158px;height:22px;overflow:hidden;"><p class="Wp-Body3-P"><span class="Wp-Hyperlink2-C Sz-Hyperlink2-C-130px"><a class="Wp-Hyperlink2-C-H" href="#" style="text-decoration:none;">results</a></span></p></div><img src="wpimages/wp2b2acbbd_06.png" border="0" width="201" height="1" alt="" style="position:absolute;left:60px;top:517px;"><img src="wpimages/wp949658e6_06.png" border="0" width="24" height="24" alt="" style="position:absolute;left:238px;top:397px;"><img src="wpimages/wp949658e6_06.png" border="0" width="24" height="24" alt="" style="position:absolute;left:238px;top:429px;"><img src="wpimages/wp949658e6_06.png" border="0" width="24" height="24" alt="" style="position:absolute;left:238px;top:459px;"><img src="wpimages/wp949658e6_06.png" border="0" width="24" height="24" alt="" style="position:absolute;left:238px;top:492px;"><img src="wpimages/wpb9bd76de_06.png" border="0" width="20" height="18" alt="" style="position:absolute;left:304px;top:950px;"><div style="position:absolute;left:345px;top:946px;width:268px;height:27px;overflow:hidden;"><p class="Wp-Sub-Heading-2-P"><span class="Sub-Heading-2-C">RBS-<wbr>LYSIS</span></p></div><div style="position:absolute;left:345px;top:984px;width:268px;height:27px;overflow:hidden;"><p class="Wp-Sub-Heading-2-P"><span class="Sub-Heading-2-C">PLAC-<wbr>CI</span></p></div><div style="position:absolute;left:345px;top:1024px;width:268px;height:27px;overflow:hidden;"><p class="Wp-Sub-Heading-2-P"><span class="Sub-Heading-2-C">PRM-<wbr>GFP</span></p></div><div style="position:absolute;left:345px;top:1062px;width:268px;height:27px;overflow:hidden;"><p class="Wp-Sub-Heading-2-P"><span class="Sub-Heading-2-C">More...</span></p></div><img src="wpimages/wpb9bd76de_06.png" border="0" width="20" height="18" alt="" style="position:absolute;left:304px;top:987px;"><img src="wpimages/wpb9bd76de_06.png" border="0" width="20" height="18" alt="" style="position:absolute;left:304px;top:1029px;"><img src="wpimages/wpb9bd76de_06.png" border="0" width="20" height="18" alt="" style="position:absolute;left:304px;top:1064px;"><div style="position:absolute;left:661px;top:979px;width:279px;height:23px;overflow:hidden;"><p class="Wp-Sub-Heading-1-P"><span class="Sub-Heading-1-C">Shan Zhou</span></p></div><img src="wpimages/wp6f6648ba_06.png" border="0" width="285" height="43" title="" alt="Daily improvements&#10;" style="position:absolute;left:658px;top:939px;"><div style="position:absolute;left:661px;top:1002px;width:279px;height:51px;overflow:hidden;"><p class="Wp-Body3-P"><span class="Body3-C">A nonlethal concentration of H202(0.05%) greatly enhances near-<wbr>ultraviolet(NUV) inactivation    of phageT7.Simultaneous treatment with H202 and NUV reduces theamount of DNA injected    into the bacterial host, but not-<wbr>the number of phage adsorbed.Not only were recombination    and gene expression of late markers reduced up on treatment of phage&#39;11withNUVplusH202,but    also a gradient of recombination resulted,with markers injected first reduced to    a lesser extent than those injected last.Double-<wbr>strand DNA breaks were not detected</span></p></div><a href="javascript:;" id="btn_25" class="Button3" style="position:absolute;left:661px;top:1061px;width:80px;height:20px;"><span>more</span></a><img src="wpimages/wp0985b787_06.png" border="0" width="960" height="256" alt="" style="position:absolute;left:20px;top:1226px;"><div style="position:absolute;left:60px;top:1132px;width:880px;height:82px;overflow:hidden;"><p class="Feature2-P"><span class="Feature2-C">REFERENCES</span></p></div><div style="position:absolute;left:60px;top:1228px;width:434px;height:153px;overflow:hidden;"><p class="Body3-P"><span class="Body3-C">A nonlethal concentration of H202(0.05%) greatly enhances near-<wbr>ultraviolet(NUV) inactivation    of phageT7.Simultaneous treatment with H202 and NUV reduces theamount of DNA injected    into the bacterial host, but not-<wbr>the number of phage adsorbed.Not only were recombination    and gene expression of late markers reduced up on treatment of phage&#39;11withNUVplusH202,but    also a gradient of recombination resulted,with markers injected first reduced to    a lesser extent than those injected last.Double-<wbr>strand DNA breaks were not detected</span></p></div><div style="position:absolute;left:506px;top:1228px;width:434px;height:153px;overflow:hidden;"><p class="Body3-P"><span class="Body3-C">A nonlethal concentration of H202(0.05%) greatly enhances near-<wbr>ultraviolet(NUV) inactivation    of phageT7.Simultaneous treatment with H202 and NUV reduces theamount of DNA injected    into the bacterial host, but not-<wbr>the number of phage adsorbed.Not only were recombination    and gene expression of late markers reduced up on treatment of phage&#39;11withNUVplusH202,but    also a gradient of recombination resulted,with markers injected first reduced to    a lesser extent than those injected last.Double-<wbr>strand DNA breaks were not detected</span></p></div><img src="wpimages/wp6fe80323_06.png" border="0" width="215" height="207" alt="" style="position:absolute;left:53px;top:173px;"><div id="slider_24" style="position:absolute;left:304px;top:180px;width:636px;height:336px;overflow:hidden;">    <div id="slider_24_P1" style="position:absolute;left:0px;top:0px;width:636px;height:336px;">        <img src="wpimages/wpfbe96ce0_05_06.jpg" border="0" width="635" height="336" alt="" style="position:absolute;left:1px;top:0px;">    </div>    <div id="slider_24_P2" style="position:absolute;left:0px;top:0px;width:636px;height:336px;visibility:hidden;">        <img src="wpimages/wp4332fdec_06.png" border="0" width="224" height="336" alt="" style="position:absolute;left:1px;top:0px;">        <div style="position:absolute;left:19px;top:47px;width:184px;height:242px;overflow:hidden;">        <h3 class="Wp-Heading-322-P" style="margin-top:0px"><span class="Heading-322-C">What do we want to do </span></h3>        <p class="Wp-Body-12-P"><span class="Body-12-C-C0"><br></span></p>        <p class="Wp-Normal-_Web_-P"><span class="Normal-_Web_-C-C0">T</span><span class="Normal-_Web_-C-C1">his year, USTC-<wbr>China iGEMers are providing a novel immune system for engineering            bacteria to fight against the bacteriophage. We hope this artificial line of defeat            with gene circuits can be of practical use in fermentation industry. </span></p>        <p class="Wp-Body-12-P"><span class="Body-12-C-C0"><br></span></p>        </div>    </div></div><div style="position:absolute;left:535px;top:176px;">    <script type="text/javascript">    if(DetectFlashVer(9, 0, 0))    {    AC_FL_RunContent(        "src", "wpimages/slideshowpro?paramXMLPath=88f768227cc369e427391.xml",        "base", "wpimages",        "play", "true",        "loop", "true",        "width", "399",        "height", "359",        "salign", "C",        "scale", "ShowAll",        "id", "slidegallery_1",        "quality", "High",        "wmode", "transparent",        "allowFullScreen", "true",        "name", "slidegallery_1",        "type", "application/x-shockwave-flash",        'codebase', 'http://fpdownload.macromedia.com/get/flashplayer/current/swflash.cab',        "pluginspage", "http://www.macromedia.com/go/getflashplayer"    );    }    else    {        var alternateContent = 'An old version of the Flash plugin was detected. <strong><a href="http://www.macromedia.com/go/getflash/">Please upgrade your Flash plugin.<\/a><\/strong>';        document.write(alternateContent);    }    </script></div><img src="wpimages/wp23561e99_05_06.jpg" border="0" width="636" height="171" alt="" style="position:absolute;left:304px;top:751px;"><a href="index.html" id="btn_35" class="Button4" style="position:absolute;left:814px;top:1531px;width:84px;height:23px;"><span>Home</span></a><script type="text/javascript">var $slider_24 = new $.fn.wpslider( { g_nDivId:'#slider_24', g_nTransitStyle:9, g_nTransitTime:2000, g_bPlayAtStart:true, g_nOverlaySlidePos:2, g_nOverlaySlideMargin:20 } );</script></div></body></html>
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<a href="http://www.htmldog.com/">HTML Dog</a>
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<p>Jump straight to the <a href="#plant">plant</a>
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<h1>How to make a falafel</h1>
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<plant cite="http://www.htmldog.com/">
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<p style= "color:#6C6;">Buy a falafel seed and plant it in your garden in <abbr title="National Aeronautics and Space Administration">NASA</abbr>.</p>
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<h1>How to make a <em>falafel</em></<h1>
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<p class="chair">Etc....ipsum...schmipsum.</p>
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Revision as of 06:24, 1 August 2012

Project

COMPANY NAME

www.ustc.edu.cn

Home Project description Project Human practice
Backgrounds

Since the fermentation industry occurred, the phage infection has become a big threat to engineering bacteria. During mass production of dairy products, enzymes, amino acids, antibiotics and pesticides, the results are unimaginable if the bacterias are infected by bacteriophages . The bacterias will die abnormally and the infection will spread quickly. Without any efficient action, tremendous loss will lie ahead. Nowadays, common strategies for avoiding this catastrophe is to control the conditions of the fermentation strictly. Also, people keep selecting strains against phages. But all the strategies are time consuming and far from once for all.

However, the engineering bacteria 2012 USTC-China iGEMers hope to cultivate both protecting themselves when bacteriophage invades the strain and focusing on producing products without consuming extra energy when their living environment is safe. In this case, the quality of fermented products can be guaranteed and the profits of fermentation industry can be enormously improved.


Abstract

Experiment plan

Modeling

results

RBS-LYSIS

PLAC-CI

PRM-GFP

More...

Shan Zhou

Daily improvements

A nonlethal concentration of H202(0.05%) greatly enhances near-ultraviolet(NUV) inactivation of phageT7.Simultaneous treatment with H202 and NUV reduces theamount of DNA injected into the bacterial host, but not-the number of phage adsorbed.Not only were recombination and gene expression of late markers reduced up on treatment of phage'11withNUVplusH202,but also a gradient of recombination resulted,with markers injected first reduced to a lesser extent than those injected last.Double-strand DNA breaks were not detected

more

REFERENCES

A nonlethal concentration of H202(0.05%) greatly enhances near-ultraviolet(NUV) inactivation of phageT7.Simultaneous treatment with H202 and NUV reduces theamount of DNA injected into the bacterial host, but not-the number of phage adsorbed.Not only were recombination and gene expression of late markers reduced up on treatment of phage'11withNUVplusH202,but also a gradient of recombination resulted,with markers injected first reduced to a lesser extent than those injected last.Double-strand DNA breaks were not detected

A nonlethal concentration of H202(0.05%) greatly enhances near-ultraviolet(NUV) inactivation of phageT7.Simultaneous treatment with H202 and NUV reduces theamount of DNA injected into the bacterial host, but not-the number of phage adsorbed.Not only were recombination and gene expression of late markers reduced up on treatment of phage'11withNUVplusH202,but also a gradient of recombination resulted,with markers injected first reduced to a lesser extent than those injected last.Double-strand DNA breaks were not detected

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