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Team:St Andrews/Procedure
From 2012.igem.org
(Difference between revisions)
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<BR> </BR> | <BR> </BR> | ||
| - | <p><span class="label"><a href="#Primers2"><font color="white">Primers</font></a></span><span class="label"><a href="#Transformation2"><font color="white">Transformation</font></a></span></p> | + | <p><span class="label"><a href="#Primers2"><font color="white">Primers</font></a></span><span class="label"><a href="#Digestion2"><font color="white">Digestion</font></a></span><span class="label"><a href="#Transformation2"><font color="white">Transformation</font></a></span></p> |
<BR> </BR> | <BR> </BR> | ||
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<li><strong>GST Forward</strong> and <strong>Pd Reverse</strong></li> | <li><strong>GST Forward</strong> and <strong>Pd Reverse</strong></li> | ||
<li><strong>GST Forward</strong> and <strong>Pt Reverse</strong></li> | <li><strong>GST Forward</strong> and <strong>Pt Reverse</strong></li> | ||
| + | |||
| + | <BR> </BR> | ||
| + | <a name="Digestion2"><span class="label">Digestion</span></a> | ||
| + | |||
| + | <BR> </BR> | ||
| + | <p>pGEX-6P-1 was cut using EcoR1(954) and Xho1 (975)</p> | ||
<BR> </BR> | <BR> </BR> | ||
Revision as of 14:51, 27 July 2012
Lab Book
Procedure
- date
- what we did
- date
- what we did
- date
- what we did
anyone really keen to write this up, up to protein visualization? Don't forget to mention the differing restriction enzymes and vectors of each team. I can add in links to the protocols page.
Please also refer to our Protocols page.
Sequences Primers Sequencing results Lipid analysis
Start from lipid extraction
primers, sequence results
Sequences
who knows what those Metal Mickies are up to. Eating nachos in the Whey Pat most likely. MEGA LOLS (all caps, with a Z).
PrimersDigestionTransformation
Primers
All primers are notated 5' to 3'.
