Team:St Andrews/Procedure

From 2012.igem.org

(Difference between revisions)
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       <p>primers, sequence results</p>
       <p>primers, sequence results</p>
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<h4>Sequences</h4>
 
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<div class="btn-toolbar">
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<div class="btn-group=">
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<button class="btn-class">Sequences</button>
   <a class="btn dropdown-toggle" data-toggle="dropdown" href="#">
   <a class="btn dropdown-toggle" data-toggle="dropdown" href="#">
     Δ12 T. Cruzi
     Δ12 T. Cruzi
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   </a>
   </a>
   <ul class="dropdown-menu">
   <ul class="dropdown-menu">
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  <div class="row">
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    <div class="span4 offset4">
     atgtcgcgggttgacaatttaactgtcgcgccgggtccaccggatgtcatgaaggcggtg
     atgtcgcgggttgacaatttaactgtcgcgccgggtccaccggatgtcatgaaggcggtg
cttaagtctgggcgcaacaaggagcacaatgtcattgtgccttcctccgcactgaccatt
cttaagtctgggcgcaacaaggagcacaatgtcattgtgccttcctccgcactgaccatt
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attacggatgagaggtcactgtggaagatgctctgggaacagcgtagagagtgccgctat
attacggatgagaggtcactgtggaagatgctctgggaacagcgtagagagtgccgctat
gttgttcccgcagagggcgtctgtgtctttcatgggtaa
gttgttcccgcagagggcgtctgtgtctttcatgggtaa
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</div>
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</div>
   </ul>
   </ul>
</div>
</div>
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   </a>
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   <ul class="dropdown-menu">
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   atgactgccacgattcccccgttgacaccaacggtaacgcccagcaatcccgatcgcccg
   atgactgccacgattcccccgttgacaccaacggtaacgcccagcaatcccgatcgcccg
attgcggatctcaaactacaagacatcattaaaaccctgcccaaggaatgcttcgagaaa
attgcggatctcaaactacaagacatcattaaaaccctgcccaaggaatgcttcgagaaa
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tttaactggcaattaatgcaacaaattagtgggcaatgtcatttatatgaccccgaacat
tttaactggcaattaatgcaacaaattagtgggcaatgtcatttatatgaccccgaacat
ggctaccgcaccttcggctccctgaaaaaagtttaa
ggctaccgcaccttcggctccctgaaaaaagtttaa
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</div>
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</div>
   </ul>
   </ul>
</div>
</div>
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   </a>
   </a>
   <ul class="dropdown-menu">
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  <div class="row">
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    <div class="span4 offset4">
     gtgcgtctagaaatttcatcgcctcaaacaaagcttccttaccccaaaactgaagaatta
     gtgcgtctagaaatttcatcgcctcaaacaaagcttccttaccccaaaactgaagaatta
ccatttaccctccaagagctcagaaacgctattccagcggattgttttgagccatcggta
ccatttaccctccaagagctcagaaacgctattccagcggattgttttgagccatcggta
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gacgagccaatttggcaagctttttttaagtcctactgggcttgccattttgttcctaat
gacgagccaatttggcaagctttttttaagtcctactgggcttgccattttgttcctaat
caaggttcaggggtctattaccaatccccatccaatggtggatatcaaaagaaaccttaa
caaggttcaggggtctattaccaatccccatccaatggtggatatcaaaagaaaccttaa
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</div>
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</div>
   </ul>
   </ul>
</div>
</div>
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   </a>
   </a>
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     atgctaacagcggaaagaattaaatttacccagaaacgggggtttcgtcgggtactaaac
     atgctaacagcggaaagaattaaatttacccagaaacgggggtttcgtcgggtactaaac
caacgggtggatgcctactttgccgagcatggcctgacccaaagggataatccctccatg
caacgggtggatgcctactttgccgagcatggcctgacccaaagggataatccctccatg
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gaaaatattattaaggatgtttgccaagagtttggtgtggaatataaagtttatcccacc
gaaaatattattaaggatgtttgccaagagtttggtgtggaatataaagtttatcccacc
ttcaaagcggcgatcgcctctaactatcgctggctagaggccatgggcaaagcatcgtga
ttcaaagcggcgatcgcctctaactatcgctggctagaggccatgggcaaagcatcgtga
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</div>
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   </ul>
</div>
</div>
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   </a>
   </a>
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     atgaaggtcatcgtcgcttctggcccggacggtgcccgcaagcacgaggtggagctggca
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     <div class="span4 offset4">
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        atgaaggtcatcgtcgcttctggcccggacggtgcccgcaagcacgaggtggagctggca
gccaacgccacgctcgcagatctgaagaaggcctaccaacggggtgtggacgtgcaccgc
gccaacgccacgctcgcagatctgaagaaggcctaccaacggggtgtggacgtgcaccgc
aagtcgttcaaggttcccagcgcggagtcgccgctgccaggtgcggatagtggcaagctg
aagtcgttcaaggttcccagcgcggagtcgccgctgccaggtgcggatagtggcaagctg
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gcgaagaagaagcaccggggctacgtcaaggcggacccggccaataaaaagaaggccgcc
gcgaagaagaagcaccggggctacgtcaaggcggacccggccaataaaaagaaggccgcc
attctgcccttcatcatgtag
attctgcccttcatcatgtag
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    </div>
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</div>
   </ul>
   </ul>
</div>
</div>
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<p></p>
       <dl class="dl-horizontal">   
       <dl class="dl-horizontal">   
         <dt>date</dt>   
         <dt>date</dt>   

Revision as of 10:42, 25 July 2012

St Andrews iGEM '12

Lab Book

Procedure

anyone really keen to write this up, up to protein visualization? Don't forget to mention the differing restriction enzymes and vectors of each team. I can add in links to the protocols page.

Please also refer to our Protocols page.

Start from lipid extraction

primers, sequence results

Δ12 T. Cruzi
    atgtcgcgggttgacaatttaactgtcgcgccgggtccaccggatgtcatgaaggcggtg cttaagtctgggcgcaacaaggagcacaatgtcattgtgccttcctccgcactgaccatt ggagaaattcaggagaaaatcccggtaaagtactttgaacgcaacactacccggagcgtc atgtttcttttacgcgacctcgcacaggtggcgttgacgtacgccattatgtacgccgtc gccttgccacttgccacttccatggaagtttctgcagcaagaacgttggccgacggtggg gtgttatcattgatggcaggtacagccatgacgacagcggcgtggcttttaaaggcagtg ttgtgggcggtgttttggtttgtacagggacttaacggcactgcgctttgggtcctggct cacgaatgcggccatcaggcgttcagccccatgaaaggtttgaatgacgccgttggcatg attttgcactctgcgctgctcgtgccgtaccacagctggcgcatcacccacggcggccac cacaaacacacgaatcacctcacgaaggatcttgtgtttgttccagacaaacgaaacgcg gttgtggagctcgtggaggaggcgccgctggtgttattaattcaattattgctgattttt ctctttggttggccggcacatcttctttttaatgcctctggacaggaatttggccgactc gcgagccactttgaccccggcgctccatttttccgcagcgaagaccgtcacgatattgtc ctgtcgaatgttgggattgtcagcgcgttatttgtcattttctccagcgtttaccgcttt ggttttacaaatgttttctgctggtacattgtaccgtacctctgggtgaacttttggctt gtgtacattacatacctgcagcacacggatatacgcattcctcactacacacatgagcac tggacgtttgttcgcggtgcattggcggctgtggacagggactacggctttgtccttaac acatggctccatcacatcaatgattcccacgtggtacatcacctctttagcaaaatgcca cactacaacgcaatccaggtgacaagaaagtacattcgtgagatactgggtgccacatac attacggatgagaggtcactgtggaagatgctctgggaacagcgtagagagtgccgctat gttgttcccgcagagggcgtctgtgtctttcatgggtaa
Δ12 Synechocystis
    atgactgccacgattcccccgttgacaccaacggtaacgcccagcaatcccgatcgcccg attgcggatctcaaactacaagacatcattaaaaccctgcccaaggaatgcttcgagaaa aaagcgagcaaagcctgggcttctgttttgattaccctaggggcgatcgccgtgggctat ttgggcattatttatctgccctggtactgcttgcccattacctggatctggacagggaca gccttaacgggggccttcgttgtcggccatgactgtggccatcgctcctttgctaaaaaa cgctgggtcaatgatttagtgggacatatcgcttttgctcccctcatctaccctttccat agctggcgcctactccacgaccaccatcacctccacaccaacaaaattgaggttgataac gcctgggatccctggagtgtggaagctttccaagccagcccggcgatcgtccggcttttt tatcgggccatccggggtcccttctggtggactggttccattttccattggagcttaatg cacttcaaactttccaactttgcccaaagggaccgcaataaagtcaaattatccattgcc gttgtcttcctgtttgcggcgatcgcctttcctgccctaattatcaccacaggggtgtgg ggtttcgtcaaattttggctaatgccctggttggtgtatcacttttggatgagcactttt accattgtgcaccacaccattcccgaaattcgtttccgtcccgccgccgattggagtgcc gccgaagcccagttaaatggtactgttcactgcgattatccccgttgggtggaagtgctc tgccatgacatcaacgtccatattccccaccacctctccgttgccatcccttcctataac ctacgactagcccacggaagtttaaaagaaaactggggaccttttctttacgagcgcacc tttaactggcaattaatgcaacaaattagtgggcaatgtcatttatatgaccccgaacat ggctaccgcaccttcggctccctgaaaaaagtttaa
Δ15 Synechocystis
    gtgcgtctagaaatttcatcgcctcaaacaaagcttccttaccccaaaactgaagaatta ccatttaccctccaagagctcagaaacgctattccagcggattgttttgagccatcggta gtccggtccttgggctacttttttttggatgttggtttaattgccgggttttatgctcta gcggcctaccttgattcctggttcttctatccgattttttggttaattcagggaacccta ttctggtccctgtttgtggtgggccatgattgtggccatggctccttttccaaatccaaa acccttaataattggattggtcatctcagccacacgccaattttggtgccttaccatggc tggcgtattagtcatcgtactcaccatgccaacacgggcaatatcgacaccgacgaaagt tggtatccagtgtcggagcaaaaatataaccaaatggcctggtatgaaaaacttctacgt ttttacttgcctctgatcgcctaccccatttatctatttcggcgatcgccaaaccggcaa ggctcccatttcatgcccggcagtcccctattccgtcccggagaaaaagcagctgttctc accagcacctttgcccttgcagcctttgtcggcttccttggctttttaacttggcaattt ggctggctatttttgctgaaattttatgttgccccctacctcgtgtttgtggtgtggtta gatttggtcacatttttacatcacactgaagacaatatcccttggtatcgtggtgatgac tggtattttctcaaaggtgccctctccaccattgatcgggattacggcttcattaacccc attcaccatgacattggcacccacgtcgcccaccatattttctcgaatatgccccactac aagttacgccgggcgactgaagccatcaagcccattttaggggaatattatcgatattct gacgagccaatttggcaagctttttttaagtcctactgggcttgccattttgttcctaat caaggttcaggggtctattaccaatccccatccaatggtggatatcaaaagaaaccttaa
Δ6 Synechocystis
    atgctaacagcggaaagaattaaatttacccagaaacgggggtttcgtcgggtactaaac caacgggtggatgcctactttgccgagcatggcctgacccaaagggataatccctccatg tatctgaaaaccctgattattgtgctctggttgttttccgcttgggcctttgtgcttttt gctccagttatttttccggtgcgcctactgggttgtatggttttggcgatcgccttggcg gccttttccttcaatgtcggccacgatgccaaccacaatgcctattcctccaatccccac atcaaccgggttctgggcatgacctacgattttgtcgggttatctagttttctttggcgc tatcgccacaactatttgcaccacacctacaccaatattcttggccatgacgtggaaatc catggagatggcgcagtacgtatgagtcctgaacaagaacatgttggtatttatcgtttc cagcaattttatatttggggtttatatcttttcattcccttttattggtttctctacgat gtctacctagtgcttaataaaggcaaatatcacgaccataaaattcctcctttccagccc ctagaattagctagtttgctagggattaagctattatggctcggctacgttttcggctta cctctggctctgggcttttccattcctgaagtattaattggtgcttcggtaacctatatg acctatggcatcgtggtttgcaccatctttatgctggcccatgtgttggaatcaactgaa tttctcacccccgatggtgaatccggtgccattgatgacgagtgggctatttgccaaatt cgtaccacggccaattttgccaccaataatcccttttggaactggttttgtggcggttta aatcaccaagttacccaccatcttttccccaatatttgtcatattcactatccccaattg gaaaatattattaaggatgtttgccaagagtttggtgtggaatataaagtttatcccacc ttcaaagcggcgatcgcctctaactatcgctggctagaggccatgggcaaagcatcgtga
ELO 6 L. major
    atgaaggtcatcgtcgcttctggcccggacggtgcccgcaagcacgaggtggagctggca gccaacgccacgctcgcagatctgaagaaggcctaccaacggggtgtggacgtgcaccgc aagtcgttcaaggttcccagcgcggagtcgccgctgccaggtgcggatagtggcaagctg cgcccgaacctcattactctgtcagataaggtgcccctgtcgcagcagggggtgaaggat ggctcggtgatcacttacaaggacctcggcccgcagatcggctaccgcacggtgttctac gtcgagtatgccggccccatcgccttcatgctgctgtacgccatgcgcccttcgctcatc tacggctctgccccgatgccggcttacggctacacgcagaagctatacattggcctcttc ctcgcccacttcttaaagcgcgagctcgagtccatgtttgtgcacaagttctcgcaccca acgatgccgatgcgcaacatcttcaagaactgcatctactactggtccttcgccgccttc atcggctacgtgctgtgcagtccttcattcacgccgaccagcaccatgcagtcaaacttc ggcgccgtggtcatggtcatcaacgagctgctgaacttcgcggtgcactaccagcttagc acgatgcgcaagtccgatggtgacaccacccgcaacgtgccgcaaggccctctgttcgcc ttcgtctcgtgcccgaactacttctttgagattatgtcgtgggtgtccttttccatcggc acaaatatgttatcctcctggttcttcacactcgccggtttcgtgcagatggcggactgg gcgaagaagaagcaccggggctacgtcaaggcggacccggccaataaaaagaaggccgcc attctgcccttcatcatgtag

date
what we did

who knows what those Metal Mickies are up to. Eating nachos in the Whey Pat most likely.

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University of St Andrews, 2012.

Contact us: igem2012@st-andrews.ac.uk, Twitter, Facebook

This iGEM team has been funded by the MSD Scottish Life Sciences Fund. The opinions expressed by this iGEM team are those of the team members and do not necessarily represent those of Merck Sharp & Dohme Limited, nor its Affiliates.

Retrieved from "http://2012.igem.org/Team:St_Andrews/Procedure"