Team:Exeter/lab book/gibs/wk1
From 2012.igem.org
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- | <! | + | <!12.7.12 <p>Chose three colonies from each plate.</p> |
+ | <p>Transferred aseptically into 1:1000 ampicillin:LB broth.</p> <p>Incubated at 37*C, 220rpm overnight.</p><p>13.7.12</p> | ||
+ | <p>Cultures were transferred to identical sterile falcon tubes and centrifuged at 21 C, 3900 rcf, for 2 minutes.</p> | ||
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Revision as of 08:24, 25 July 2012
Operon Construction: 9th - 13th July 2012 Chose three colonies from each plate.Transferred aseptically into 1:1000 ampicillin:LB broth. Incubated at 37*C, 220rpm overnight. 13.7.12 Cultures were transferred to identical sterile falcon tubes and centrifuged at 21 C, 3900 rcf, for 2 minutes. |