Team:WashU/Week8
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In addition, we ran several PCRs today. | In addition, we ran several PCRs today. | ||
- | Following the NEB protocol, we ran four PCRs to amplify the CCD plasmid (PUT PROPER NAME HERE) and also tried | + | Following the NEB protocol, we ran four PCRs to amplify the CCD plasmid (PUT PROPER NAME HERE) and also tried three colony PCRs, using colonies 2, 4, and 13. However, the PCRs were all unsuccessful and resulted only in primer dimers. |
To enable amplification of our plasmid PSL2131, PsbA2, and CS42S, we designed primers to multiply the aforementioned DNA pieces. | To enable amplification of our plasmid PSL2131, PsbA2, and CS42S, we designed primers to multiply the aforementioned DNA pieces. | ||
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</html> | </html> | ||
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PCR the color constructs in order to biobrick them | PCR the color constructs in order to biobrick them |
Revision as of 20:28, 19 July 2012