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Team:Cornell/Protocols
From 2012.igem.org
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=Protocols= | =Protocols= | ||
| + | ==DNA Extraction== | ||
*[[Team:Cornell/Protocols/Miniprep|Miniprep]] | *[[Team:Cornell/Protocols/Miniprep|Miniprep]] | ||
| + | *[[Team:Cornell/Protocols/Gel_purification|Gel Purification]] | ||
| + | |||
| + | ==PCR Methods== | ||
*[[Team:Cornell/Protocols/Vent_PCR|Vent PCR]] | *[[Team:Cornell/Protocols/Vent_PCR|Vent PCR]] | ||
*[[Team:Cornell/Protocols/Phusion_PCR|Phusion PCR]] | *[[Team:Cornell/Protocols/Phusion_PCR|Phusion PCR]] | ||
*[[Team:Cornell/Protocols/Colony_PCR|Colony PCR]] | *[[Team:Cornell/Protocols/Colony_PCR|Colony PCR]] | ||
| - | + | ||
| - | + | ==Plasmid Insertion== | |
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
*[[Team:Cornell/Protocols/Transformation_ecoli|Transformation of E. coli]] | *[[Team:Cornell/Protocols/Transformation_ecoli|Transformation of E. coli]] | ||
*[[Team:Cornell/Protocols/Transformation_ shewanella|Transformation of Shewanella]] | *[[Team:Cornell/Protocols/Transformation_ shewanella|Transformation of Shewanella]] | ||
| - | *[[Team:Cornell/Protocols/ | + | *[[Team:Cornell/Protocols/Conjugation|Conjugation]] |
| - | + | ||
| + | ==Maintenance Tasks== | ||
*[[Team:Cornell/Protocols/LB|Making LB]] | *[[Team:Cornell/Protocols/LB|Making LB]] | ||
*[[Team:Cornell/Protocols/Autoclave|Autoclave]] | *[[Team:Cornell/Protocols/Autoclave|Autoclave]] | ||
*[[Team:Cornell/Protocols/SOB|Making Super Optimal Broths]] | *[[Team:Cornell/Protocols/SOB|Making Super Optimal Broths]] | ||
| - | |||
*[[Team:Cornell/Protocols/Glycerol_stocks|Preparing Glycerol Stocks]] | *[[Team:Cornell/Protocols/Glycerol_stocks|Preparing Glycerol Stocks]] | ||
*[[Team:Cornell/Protocols/Overnight_cultures|Preparing Overnight Cultures]] | *[[Team:Cornell/Protocols/Overnight_cultures|Preparing Overnight Cultures]] | ||
| + | |||
| + | ==DNA Assessment== | ||
| + | *[[Team:Cornell/Protocols/Gel_electrophoresis|Gel Electrophoresis]] | ||
| + | *[[Team:Cornell/Protocols/DNA_quatification|DNA Quantification]] | ||
| + | *[[Team:Cornell/Protocols/Sequencing|Sequencing]] | ||
| + | |||
| + | ==I DON"T KNOW== | ||
| + | *[[Team:Cornell/Protocols/Digestion|Digestion Reaction]] | ||
| + | *[[Team:Cornell/Protocols/Ligation|Ligation Reaction]] | ||
| + | *[[Team:Cornell/Protocols/Gibson_assembly|Gibson Assembly]] | ||
| + | *[[Team:Cornell/Protocols/Dephosphorylation|Dephosphorylation]] | ||
| + | *[[Team:Cornell/Protocols/PCR_cleanup|PCR Clean Up]] | ||
Revision as of 16:40, 19 July 2012
| Home | Team | Project | Parts | Modeling | Notebook | Protocols | Safety | Attributions |
|---|
Contents |
Protocols
DNA Extraction
PCR Methods
Plasmid Insertion
Maintenance Tasks
- Making LB
- Autoclave
- Making Super Optimal Broths
- Preparing Glycerol Stocks
- Preparing Overnight Cultures
DNA Assessment
I DON"T KNOW
