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Team:Bordeaux/Third
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Revision as of 14:46, 20 July 2012
Day 10 : 16-07-2012
This week we decided to make our own competent cells Regarding the bad/average results we had with our commercial DH5α and XL1blue we decided to try with DH10B strain We followed the protocol proposed by the patregistry.org Read Protocol
Day 11 : 18-07-2012
The competence test gave good results with a good amount of colonies on each plates and no colonies on the control plate. Today we will transform DNA for every biobrick left. We will use our new competent cells.
We will proceed by heat-shock as described in the partregistry.org protocol Read Protocol
Day 12 : 19-07-2012
