Team:Cornell/Notebook
From 2012.igem.org
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*[[Team:Cornell/Notebook/Arsenic reporter|Arsenic reporter]] -- test a system for Shewanella detecting arsenates and activating the [[Team:Cornell/Project/Mtr pathway|Mtr pathway]] to produce an electrical current | *[[Team:Cornell/Notebook/Arsenic reporter|Arsenic reporter]] -- test a system for Shewanella detecting arsenates and activating the [[Team:Cornell/Project/Mtr pathway|Mtr pathway]] to produce an electrical current |
Revision as of 19:12, 19 July 2012
Home | Team | Project | Parts | Modeling | Notebook | Protocols | Safety | Attributions |
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Wetlab Notebook
Above is the link to the notebook kept by all of our team members working in the lab with biological parts. This subteam consists of Claire, Dylan, Steven, Spencer, Caleb, Rafaelle, Danielle, Youjin, Mark, Tina, Chie, Swati, and Shweta.
Drylab Notebook
Above is the link to the notebook kept by all of our team members working on device designs, math modeling, software programming, etc. This subteam consists of Dan, Maneesh, Manny, Robert, Lydia, Kelvin, Tina, and Chie.
Outreach Notebook
Above is the link the notebook kept by the coordinators of our team's outreach events. Many team members participate in outreach events, but they are all coordinated by Swati and Shweta.
Strain List
Above is the link to our spreadsheet of our organism strains, including name abbreviations used throughout the notebook and any relevent data on them such as the contained plasmid.
Old Links
DO NOT DO ANY EDITING ON THE BELOW LINKS. THE BETTER VERSIONS ARE THE ABOVE. THESE ARE ONLY STILL HERE FOR REFERENCE AS A "JUST IN CASE"
Subprojects
- Arsenic reporter -- test a system for Shewanella detecting arsenates and activating the Mtr pathway to produce an electrical current
- nah operon -- an operon that converts napthalene into salicylate, allowing it to be detected by the salicylate reporter.
- Salicylate reporter -- a system for Shewanella detecting salicylate and activating the Mtr pathway to produce an electrical current
Protocols
- Miniprep
- Vent PCR
- Phusion PCR
- Colony PCR
- Gel Electrophoresis
- Gel Purification
- DNA Quantification
- Digestion Reaction
- Dephosphorylation
- PCR Clean Up
- Ligation Reaction
- Transformation of E. coli
- Transformation of Shewanella
- Sequencing
- Gibson Assembly
- Making LB
- Autoclave
- Making Super Optimal Broths